{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:45:17Z","timestamp":1789195517466,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,7,1]],"date-time":"2016-07-01T00:00:00Z","timestamp":1467331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:title>New Findings<\/jats:title><jats:sec><jats:label\/><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>We investigated whether intestinal vagal afferents are necessary for the insulinotropic effect of glucagon\u2010like peptide\u20101 (GLP\u20101) infused into a mesenteric artery or a peripheral vein before and after acute truncal vagotomy.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>We found no effect of truncal vagotomy on the insulinotropic effect of exogenous GLP\u20101 and speculate that high circulating concentrations of GLP\u20101 after <jats:sc>i.v<\/jats:sc>. and <jats:sc>i.a<\/jats:sc>. infusion might have overshadowed any neural signalling component. We propose that further investigations into the possible vagal afferent signalling of GLP\u20101 would best be pursued using enteral stimuli to provide high subepithelial levels of endogenous GLP\u20101.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>Glucagon\u2010like peptide 1 (GLP\u20101) is secreted from the gut in response to luminal stimuli and stimulates insulin secretion in a glucose\u2010dependent manner. As a result of rapid enzymatic degradation of GLP\u20101 by dipeptidyl peptidase\u20104, a signalling pathway involving activation of intestinal vagal afferents has been proposed. We conducted two series of experiments in \u03b1\u2010chloralose\u2010anaesthetized pigs. In protocol\u00a0I, pigs (<jats:italic>n<\/jats:italic>\u00a0=\u00a014) were allocated for either <jats:sc>i.v<\/jats:sc>. or <jats:sc>i.a<\/jats:sc>. (mesenteric) GLP\u20101 infusions (1 and 2\u00a0pmol\u00a0kg<jats:sup>\u22121<\/jats:sup>\u00a0min<jats:sup>\u22121<\/jats:sup>, 30\u00a0min) while maintaining permissive glucose concentrations at 6\u00a0mmol\u00a0l<jats:sup>\u22121<\/jats:sup> by <jats:sc>i.v<\/jats:sc>. glucose infusion. The GLP\u20101 infusions were repeated after acute truncal vagotomy. In protocol\u00a0II, pigs (<jats:italic>n<\/jats:italic>\u00a0=\u00a027) were allocated into six groups. Glucagon\u2010like peptide 1 was infused <jats:sc>i.v<\/jats:sc>. or <jats:sc>i.a<\/jats:sc>. (mesenteric) for 1\u00a0h at 3 or 30\u00a0pmol\u00a0kg<jats:sup>\u22121<\/jats:sup>\u00a0min<jats:sup>\u22121<\/jats:sup>. During the steady state (21\u00a0min into the GLP\u20101 infusion), glucose (0.2\u00a0g\u00a0kg<jats:sup>\u22121<\/jats:sup>, <jats:sc>i.v<\/jats:sc>.) was administered over 9\u00a0min to stimulate \u03b2\u2010cell secretion. Thirty minutes after the glucose infusion, GLP\u20101 infusions were discontinued. Following a washout period, the vagal trunks were severed in four of six groups (vagal trunks were left intact in two of six groups), whereupon all infusions were repeated. We found no effect of vagotomy on insulin or glucagon secretion during administration of exogenous GLP\u20101 in any experiment. We speculate that the effect of exogenous GLP\u20101 overshadowed any effect occurring via the vagus. Within dosage groups, total GLP\u20101 concentrations were similar, but intact GLP\u20101 concentrations were much lower when infused via the mesenteric artery because of extensive degradation of GLP\u20101 in the splanchnic bed. This demonstrates the effectiveness with which intestinal capillary dipeptidyl peptidase\u20104 protects the systemic circulation from intact GLP\u20101, consistent with a local role for GLP\u20101 involving activation of vagal pathways.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep085692","type":"journal-article","created":{"date-parts":[[2016,3,31]],"date-time":"2016-03-31T00:35:55Z","timestamp":1459384555000},"page":"895-912","source":"Crossref","is-referenced-by-count":6,"title":["The insulinotropic effect of exogenous glucagon\u2010like peptide\u20101 is not affected by acute vagotomy in anaesthetized pigs"],"prefix":"10.1113","volume":"101","author":[{"given":"Simon","family":"Veedfald","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise Wulff","family":"Christensen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara Agnete Hjort","family":"Larsen","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina Rahr","family":"Hj\u00f8llund","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Plamboeck","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bolette","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn Fiona","family":"Deacon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens Juul","family":"Holst","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences (endocrinology section) and NNF Center for Basic Metabolic Research (translational metabolic physiology section), Faculty of Health and Medical Sciences University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.4.R1449"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.80.3.952"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1996.271.3.E458"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jat.727"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"5807","DOI":"10.1016\/S0021-9258(19)70701-7","article-title":"Biogenesis of plasma membrane glycoproteins. 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