{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:13:43Z","timestamp":1740136423914,"version":"3.37.3"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T00:00:00Z","timestamp":1481760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81300180","31401006"],"award-info":[{"award-number":["81300180","31401006"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7151001"],"award-info":[{"award-number":["7151001"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>High titres of autoantibodies against the second extracellular loop of the \u03b2<jats:sub>1<\/jats:sub>\u2010adrenergic receptor (\u03b2<jats:sub>1<\/jats:sub>\u2010AAs) can be detected in the sera of patients with ventricular arrhythmias, but a causal relationship between \u03b2<jats:sub>1<\/jats:sub>\u2010AAs and ventricular arrhythmias has not been established.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Monoclonal \u03b2<jats:sub>1<\/jats:sub>\u2010AAs (\u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs) were used in the experiments. We showed that \u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs increased susceptibility to ventricular arrhythmias and induced repolarization abnormalities. Antibody adsorption of \u03b2<jats:sub>1<\/jats:sub>\u2010AAs will be a potential new therapeutic strategy for ventricular arrhythmias in patients with high titres of \u03b2<jats:sub>1<\/jats:sub>\u2010AAs.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>High titres of autoantibodies against the second extracellular loop of the \u03b2<jats:sub>1<\/jats:sub>\u2010adrenergic receptor (\u03b2<jats:sub>1<\/jats:sub>\u2010AAs) can be detected in sera from patients with ventricular arrhythmias, but a causal relationship between \u03b2<jats:sub>1<\/jats:sub>\u2010AAs and ventricular arrhythmias has not been established. In this work, ECGs of guinea\u2010pigs and isolated guinea\u2010pig hearts were recorded. Ventricular tachycardia (VT) and ventricular fibrillation (VF) were evoked by programmed electrical stimulation of the left ventricular epicardium of isolated guinea\u2010pig hearts. The monophasic action potential and effective refractory period of the left ventricle were recorded in paced isolated guinea\u2010pig hearts. Furthermore, to increase the specificity, monoclonal autoantibodies against the second extracellular loop of the \u03b2<jats:sub>1<\/jats:sub>\u2010adrenergic receptor (\u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs) were used in all experiments. The results showed that \u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs induced premature ventricular contractions in guinea\u2010pigs and isolated guinea\u2010pig hearts. In addition, \u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs decreased the threshold of VT\/VF and prolonged the duration of VT\/VF. Furthermore, \u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs shortened the corrected QT interval and effective refractory period, and prolonged late\u2010phase repolarization of the monophasic action potential (MAPD<jats:sub>90\u221230<\/jats:sub>). These changes in electrophysiological parameters might be attributed, at least in part, to the arrhythmogenicity of \u03b2<jats:sub>1<\/jats:sub>\u2010AR mAbs.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep085778","type":"journal-article","created":{"date-parts":[[2016,12,15]],"date-time":"2016-12-15T17:13:52Z","timestamp":1481822032000},"page":"25-33","source":"Crossref","is-referenced-by-count":2,"title":["\u03b2<sub>1<\/sub>\u2010Adrenoceptor autoantibodies increase the susceptibility to ventricular arrhythmias involving abnormal repolarization in guinea\u2010pigs"],"prefix":"10.1113","volume":"102","author":[{"given":"Yu\u2010Hui","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences Capital Medical University  Beijing 100069 China"},{"name":"Department of Cardiology, Beijing Anzhen Hospital Capital Medical University  Beijing 100029 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Basic Medical Sciences Capital Medical University  Beijing 100069 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences Capital Medical University  Beijing 100069 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences Capital Medical University  Beijing 100069 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology, School of Basic Medical Sciences Shanxi Medical University  Shanxi 030001 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chang\u2010Sheng","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of 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