{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:09:59Z","timestamp":1747188599263,"version":"3.40.5"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,19]],"date-time":"2019-04-19T00:00:00Z","timestamp":1555632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC31571179","NSFC31371165"],"award-info":[{"award-number":["NSFC31571179","NSFC31371165"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>The aim was to examine and compare the contributions of caveolin\u20101 to the contractile responses mediated by L\u2010type voltage\u2010dependent calcium channels, store\u2010operated Ca<jats:sup>2+<\/jats:sup> channels and receptor\u2010operated Ca<jats:sup>2+<\/jats:sup> channels in two different types of arteries from two\u2010kidney, one\u2010clip hypertensive rats.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>We demonstrated that the density of caveolae and caveolin\u20101 expression were significantly upregulated in the aorta of two\u2010kidney, one\u2010clip hypertensive rats, but not in the third\u2010order branches of mesenteric arteries. We highlight that caveolin\u20101 plays an important role in aortic constriction by enhancing receptor\u2010operated Ca<jats:sup>2+<\/jats:sup> entry in the hypertensive rat model.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Calcium and its multiple regulatory mechanisms are crucial for the development of hypertension. Among these regulatory mechanisms, store\u2010operated Ca<jats:sup>2+<\/jats:sup> entry (SOCE) and receptor\u2010operated Ca<jats:sup>2+<\/jats:sup> entry (ROCE) mediate agonist\u2010induced calcium influx, contributing to vascular contraction. The SOCE and ROCE are regulated by a variety of mechanisms involving caveolin\u20101 (Cav1), which has been found to be strongly associated with hypertension in gene polymorphism. In the present study, we investigated the role of Cav1 during the enhanced activity of calcium channels in hypertensive arteries. We demonstrated that the expression level of Cav1 was significantly increased in the aorta of two\u2010kidney, one\u2010clip (2K1C) hypertensive rats. The disruption of caveolae by methyl\u2010\u03b2\u2010cyclodextrin did not cause a marked difference in agonist\u2010induced vasoconstriction in the third\u2010order branches of the mesenteric arteries but strongly suppressed the aortic contractile response to endothelin\u20101 in the 2K1C group, which was not found in the control group. The increase in Cav1 by introduction of Cav1 scaffolding domain enhancing peptide promoted the 1\u2010oleoyl\u20102\u2010acetyl\u2010glycerol\u2010induced ROCE in hypertensive aortic smooth muscle cells but did not enhance the cyclopiazonic acid\u2010induced SOCE. In the resistance arteries, similar changes were not observed, and no statistical changes of Cav1 expression were evident in the third\u2010order branches of the mesenteric arteries. Our results indicate that increased Cav1 expression might promote the altered [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>\u2010induced aortic vasoreactivity by enhancing ROCE and be involved in the pathogenesis of hypertension.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep086924","type":"journal-article","created":{"date-parts":[[2019,3,6]],"date-time":"2019-03-06T18:11:32Z","timestamp":1551895892000},"page":"932-945","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Increased caveolin\u20101 expression enhances the receptor\u2010operated Ca<sup>2+<\/sup> entry in the aorta of two\u2010kidney, one\u2010clip hypertensive rats"],"prefix":"10.1113","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1916-3788","authenticated-orcid":false,"given":"Fu\u2010Rong","family":"Yan","sequence":"first","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"},{"name":"Center for Molecular Diagnosis and Therapy Respiratory Medicine Center of Fujian Province Second Affiliated Hospital of Fujian Medical University  Quanzhou Fujian PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0940-8753","authenticated-orcid":false,"given":"Zhuang\u2010Li","family":"Zhu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6184-4977","authenticated-orcid":false,"given":"Yun\u2010Ping","family":"Mu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"},{"name":"Institute of Biomedical and Pharmaceutical Sciences Guangdong University of Technology  Guangzhou Guangdong PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0748-5181","authenticated-orcid":false,"given":"Xiao\u2010Ling","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9222-3719","authenticated-orcid":false,"given":"Da\u2010Cen","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9690-1257","authenticated-orcid":false,"given":"Zhi\u2010Juan","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1517-2350","authenticated-orcid":false,"given":"Long\u2010Xin","family":"Gui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; Department of Physiology and Pathophysiology School of Basic Medical Sciences Fujian Medical University  Fuzhou Fujian PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-2096","authenticated-orcid":false,"given":"Mo\u2010Jun","family":"Lin","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fujian Province Universities on Ion Channel and Signal Transduction in Cardiovascular Diseases; 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