{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T04:06:55Z","timestamp":1784347615962,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T00:00:00Z","timestamp":1552521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000274","name":"British Heart Foundation","doi-asserted-by":"publisher","award":["RG\/12\/10\/29802"],"award-info":[{"award-number":["RG\/12\/10\/29802"]}],"id":[{"id":"10.13039\/501100000274","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["HL091071"],"award-info":[{"award-number":["HL091071"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2019,5]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>What is the cellular basis of the protection conferred on the heart by overexpression of caveolin\u20103 (Cav\u20103 OE) against many of the features of heart failure normally observed <jats:italic>in vivo<\/jats:italic>?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Cav\u20103 overexpression has little effect in normal ventricular myocytes but reduces cellular hypertrophy and preserves t\u2010tubular <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>, but not local t\u2010tubular Ca<jats:sup>2+<\/jats:sup> release, in heart failure induced by pressure overload in mice. Thus Cav\u20103 overexpression provides specific but limited protection following induction of heart failure, although other factors disrupt Ca<jats:sup>2+<\/jats:sup> release.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Caveolin\u20103 (Cav\u20103) is an 18\u00a0kDa protein that has been implicated in t\u2010tubule formation and function in cardiac ventricular myocytes. During cardiac hypertrophy and failure, Cav\u20103 expression decreases, t\u2010tubule structure is disrupted and excitation\u2013contraction coupling (ECC) is impaired. Previous work has suggested that Cav\u20103 overexpression (OE) is cardio\u2010protective, but the effect of Cav\u20103 OE on these cellular changes is unknown. We therefore investigated whether Cav\u20103 OE in mice is protective against the cellular effects of pressure overload induced by 8\u00a0weeks\u2019 transverse aortic constriction (TAC). Cav\u20103 OE mice developed cardiac dilatation, decreased stroke volume and ejection fraction, and hypertrophy and pulmonary congestion in response to TAC. These changes were accompanied by cellular hypertrophy, a decrease in t\u2010tubule regularity and density, and impaired local Ca<jats:sup>2+<\/jats:sup> release at the t\u2010tubules. However, the extent of cardiac and cellular hypertrophy was reduced in Cav\u20103 OE compared to WT mice, and t\u2010tubular Ca<jats:sup>2+<\/jats:sup> current (<jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub>) density was maintained. These data suggest that Cav\u20103 OE helps prevent hypertrophy and loss of t\u2010tubular <jats:italic>I<\/jats:italic><jats:sub>Ca<\/jats:sub> following TAC, but that other factors disrupt local Ca<jats:sup>2+<\/jats:sup> release.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep087304","type":"journal-article","created":{"date-parts":[[2019,2,20]],"date-time":"2019-02-20T22:51:17Z","timestamp":1550703077000},"page":"654-666","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Cardiac\u2010specific overexpression of caveolin\u20103 preserves t\u2010tubular <i>I<\/i><sub>Ca<\/sub> during heart failure in mice"],"prefix":"10.1113","volume":"104","author":[{"given":"Cherrie H. T.","family":"Kong","sequence":"first","affiliation":[{"name":"School of Physiology Pharmacology &amp; Neuroscience Biomedical Sciences Building University of Bristol  Bristol BS8 1TD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2483-6203","authenticated-orcid":false,"given":"Simon M.","family":"Bryant","sequence":"additional","affiliation":[{"name":"School of Physiology Pharmacology &amp; Neuroscience Biomedical Sciences Building University of Bristol  Bristol BS8 1TD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy J.","family":"Watson","sequence":"additional","affiliation":[{"name":"School of Physiology Pharmacology &amp; Neuroscience Biomedical Sciences Building University of Bristol  Bristol BS8 1TD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Roth","sequence":"additional","affiliation":[{"name":"VA San Diego Healthcare System and Department of Anesthesiology University of California  San Diego, La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemal H.","family":"Patel","sequence":"additional","affiliation":[{"name":"VA San Diego Healthcare System and Department of Anesthesiology University of California  San Diego, La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark B.","family":"Cannell","sequence":"additional","affiliation":[{"name":"School of Physiology Pharmacology &amp; Neuroscience Biomedical Sciences Building University of Bristol  Bristol BS8 1TD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2035-9489","authenticated-orcid":false,"given":"Andrew F.","family":"James","sequence":"additional","affiliation":[{"name":"School of Physiology Pharmacology &amp; Neuroscience Biomedical Sciences Building University of Bristol  Bristol BS8 1TD UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-5900","authenticated-orcid":false,"given":"Clive H.","family":"Orchard","sequence":"additional","affiliation":[{"name":"School of Physiology Pharmacology &amp; Neuroscience Biomedical Sciences Building University of Bristol  Bristol BS8 1TD 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