{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T10:01:53Z","timestamp":1780999313046,"version":"3.54.1"},"reference-count":55,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T00:00:00Z","timestamp":1549929600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec>\n                    <jats:title>New Findings<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:bold>What is the central question of this study?<\/jats:bold>\n                          <\/jats:p>\n                          <jats:p>Does glucocorticoid excess disrupt brown adipose tissue (BAT) phenotype and function?<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:bold>What is the main finding and its importance?<\/jats:bold>\n                          <\/jats:p>\n                          <jats:p>Glucocorticoid excess induced an extensive remodelling of interscapular BAT, resulting in a white\u2010like phenotype in association with metabolic disturbances. Glucocorticoids might be an important modulator of BAT physiology and BAT may have a role in pathophysiology of metabolic disturbances induced by glucocorticoid excess.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      In mammals, brown adipose tissue (BAT) is centrally involved in energy metabolism. To test the hypothesis that glucocorticoid excess disrupts BAT phenotype and function, male Wistar rats were treated with corticosterone in drinking water for 21\u00a0days. To confirm induction of glucocorticoid excess and metabolic disturbances, adrenal weight, corticotrophin releasing hormone mRNA levels and corticosterone serum levels were measured and a glucose tolerance test and serum triacylglycerol analyses were performed. Adipose tissue deposits were excised, weighed and evaluated by a set of biochemical, histological and molecular procedures, including thin\u2010layer chromatography, histochemistry, immunohistochemistry, quantitative real\u2010time polymerase chain reaction, high\u2010resolution oxygraphy, ATP synthesis and enzymatic activity measurements. The approach was successful in induction of glucocorticoid excess and metabolic disturbances. Lower body weight and increased adiposity were observed in corticosterone\u2010treated rats. Interscapular brown adipose tissue (iBAT) showed higher sensitivity to glucocorticoids than other fat deposits. The treatment induced lipid accumulation, unilocular rearrangement, increased collagen content and decreased innervation in iBAT. Furthermore, expression of\n                      <jats:italic>Prdm16<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;\u00a00.05),\n                      <jats:italic>Ucp1<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05) and\n                      <jats:italic>Slc7a10<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05) mRNA decreased, while expression of\n                      <jats:italic>Fasn<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05) and\n                      <jats:italic>Lep<\/jats:italic>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05) mRNA increased in brown adipose tissue. Also, the levels of UCP1 diminished (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.001, 2.5\u2010fold). Finally, lower oxygen consumption (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05), ATP synthesis (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05) and mitochondrial content (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0&lt;0.05) were observed in iBAT of glucocorticoid\u2010treated rats. Glucocorticoid excess induced an extensive remodelling of interscapular brown adipose tissue, resulting in a white\u2010like phenotype in association with metabolic disturbances.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/ep087332","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T13:54:28Z","timestamp":1547733268000},"page":"514-528","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Brown adipose tissue remodelling induced by corticosterone in male Wistar rats"],"prefix":"10.1113","volume":"104","author":[{"given":"Felippe","family":"Mousovich\u2010Neto","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Fisiologia End\u00f3crina Doris Rosenthal Universidade Federal do Rio de Janeiro  Rio de Janeiro Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Souza","family":"Matos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Fisiologia End\u00f3crina Doris Rosenthal Universidade Federal do Rio de Janeiro  Rio de Janeiro Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Carolina Rego","family":"Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroqu\u00edmica Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Augusto","family":"de Melo Reis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Neuroqu\u00edmica Instituto de Biof\u00edsica Carlos Chagas Filho Universidade Federal do Rio de Janeiro  Rio de Janeiro Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia Correa","family":"Atella","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Bioqu\u00edmica de Lip\u00eddeos e 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