{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:10:06Z","timestamp":1747188606541,"version":"3.40.5"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2019,4,22]],"date-time":"2019-04-22T00:00:00Z","timestamp":1555891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004681","name":"Higher Education Commission, Pakistan","doi-asserted-by":"publisher","award":["117\u20106027\u2010PS7\u2010076"],"award-info":[{"award-number":["117\u20106027\u2010PS7\u2010076"]}],"id":[{"id":"10.13039\/501100004681","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>What is the mechanism of wheat\u2010induced pulmonary inflammation and how does a hydrazide derivative modulate it?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>A hydrazide derivative significantly reduced wheat\u2010induced pulmonary inflammation in a rat model mainly by down\u2010regulating inflammatory cell infiltration, pathological lesions in the lungs and the level of pro\u2010inflammatory cytokines, COX\u20101, COX\u20102 and T\u2010cell proliferation.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>We investigated the ameliorative anti\u2010inflammatory effect of a previously synthesized hydrazide derivative (<jats:italic>N<\/jats:italic>\u2032\u2010(4\u2010methoxybenzylidene)\u20106\u2010(4\u2010chlorophenyl)\u20103\u2010methyl\u20101\u2010phenyl\u20101<jats:italic>H<\/jats:italic>\u2010pyrazolo[3,4\u2010b]pyridine\u20104\u2010carbohydrazide; MD) as an immunomodulator in a newly developed allergen\u2010induced pulmonary inflammation (AIPI) rat model. Wheat and thresher dust were used as allergens to induce pulmonary inflammation while MD was used to reverse the inflammatory response. Blood and bronchoalveolar lavage fluid (BALF) were collected after killing the rats and inflammatory cells were counted. Histological analysis of lung airways was carried out by haematoxylin and eosin and periodic acid\u2013Schiff staining while the level of total serum IgE, interleukin (IL)\u20104, IL\u20105 and cyclooxygenase (COX)\u20101 in BALF and <jats:italic>in vitro<\/jats:italic> T\u2010cell proliferation in spleen were measured through enzyme\u2010linked immunosorbent assay. mRNA expression level of IL\u20104, IL\u20105, IL\u201013, transforming growth factor \u03b2 (TGF\u2010\u03b2), interferon\u2010\u03b3, tumour necrosis factor \u03b1, COX\u20101 and COX\u20102 was evaluated by qRT\u2010PCR. A liver and kidney function test was used to observe any toxic impact of MD. The results indicated that 2\u00a0mg of wheat and thresher dust led to higher levels of inflammatory cytokines in the blood, BALF and lung airways of rats. MD potentially down\u2010regulated the inflammatory cell infiltration in BALF and pathological lesions in the lung airways of AIPI rats. MD significantly suppressed the elevated total serum IgE, along with IL\u20104, IL\u20105, IL\u201013, TGF\u2010\u03b2, COX\u20101 and COX\u20102 mRNA expression and T\u2010cell proliferation in spleen. In conclusion, MD at 10\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup> exhibited a significant reduction in all the markers in both wheat\u2010 and thresher dust\u2010induced pulmonary inflammation mainly by inhibiting pro\u2010inflammatory cytokine production and T\u2010cell proliferation. The data suggest that inhibition of the T\u2010cell response may be responsible for the modulative effect of MD in an AIPI rat model.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep087397","type":"journal-article","created":{"date-parts":[[2019,3,24]],"date-time":"2019-03-24T09:14:22Z","timestamp":1553418862000},"page":"896-919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Modulative effect of a new hydrazide derivative on wheat\u2010induced pulmonary inflammation in rats"],"prefix":"10.1113","volume":"104","author":[{"given":"Sara","family":"Siddiqui","sequence":"first","affiliation":[{"name":"Institute of Chemistry University of The Punjab  New Campus Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malka M.","family":"Samra","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of The Punjab  New Campus Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Nawaz","sequence":"additional","affiliation":[{"name":"Department of Microbiology Faculty of Veterinary Sciences University of Veterinary &amp; Animal Sciences  Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad","family":"Shahid","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of The Punjab  New Campus Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayesha","family":"Ramzan","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of The Punjab  New Campus Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Makshoof","family":"Athar","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of The Punjab  New Campus Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4242-0025","authenticated-orcid":false,"given":"Muhammad Asim Raza","family":"Basra","sequence":"additional","affiliation":[{"name":"Institute of Chemistry University of The Punjab  New Campus Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,4,22]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"14","article-title":"Prevalence of allergic disease and related allergens in Pakistan in 2007","volume":"25","author":"Ahmad F.","year":"2011","journal-title":"Journal of Postgraduate Medical Institute"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2014.03.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1586\/17476348.1.1.65"},{"key":"e_1_2_9_5_1","first-page":"1165","article-title":"Asthma exacerbations: Origin, effect, and prevention","volume":"128","author":"Apter A. 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