{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T03:05:35Z","timestamp":1775012735797,"version":"3.50.1"},"reference-count":80,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,22]],"date-time":"2020-05-22T00:00:00Z","timestamp":1590105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/R502364\/1"],"award-info":[{"award-number":["MR\/R502364\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/P021220\/1"],"award-info":[{"award-number":["MR\/P021220\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the topic of this review?<\/jats:bold>\n<\/jats:p><jats:p>This review discusses the application of new stable isotope tracer techniques in understanding the control of skeletal muscle mass.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What advances does it highlight?<\/jats:bold><\/jats:p>\n<jats:p>This review highlights current advances in stable isotope tracer techniques through their combination with high\u2010throughput proteomics technologies.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Beyond its primary locomotory and key structural functions, skeletal muscle provides additional vital roles for maintenance of metabolic health, acting as a storage point for glucose and intramuscular lipids for energy production, alongside being the largest reservoir for amino acids in the body. Therefore, maintenance of muscle mass is key to the promotion of health and well\u2010being across the lifespan and in several disease states. As such, when skeletal muscle is lost, in either clinical (cancer, organ failure etc.) or non\u2010clinical (ageing, inactivity) situations, there are potentially devastating consequences attached, with robust links existing between muscle mass loss and mortality. Great efforts are being made to reverse or slow muscle mass declines in health and disease, through combinations of lifestyle changes and nutritional and\/or pharmaceutical intervention. However, despite this comprehensive research effort, the underlying metabolic and molecular mechanisms have yet to be defined properly. However, with the rapid acceleration of analytical developments over recent years, the application of stable isotope tracers to the study of human muscle metabolism is providing unique insights into the mechanisms controlling skeletal muscle loss and allowing more targeted therapeutic strategies to be developed. The aim of this review is to highlight the technical breakthroughs in our understanding of muscle wasting in health and disease and how future directions and developments incorporating \u2018omics\u2019 with stable isotope tracers will allow for a more personalized and stratified therapeutic approach.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep087492","type":"journal-article","created":{"date-parts":[[2020,5,3]],"date-time":"2020-05-03T04:28:20Z","timestamp":1588480100000},"page":"1081-1089","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Contemporary stable isotope tracer approaches: Insights into skeletal muscle metabolism in health and disease"],"prefix":"10.1113","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9245-3095","authenticated-orcid":false,"given":"Matthew S.","family":"Brook","sequence":"first","affiliation":[{"name":"MRC\u2010Versus Arthritis Centre for Musculoskeletal Ageing Research, National Institute for Health Research Nottingham Biomedical Research Centre University of Nottingham  Nottingham UK"},{"name":"School of Life Science Queen's Medical Centre  Nottingham UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8808-8243","authenticated-orcid":false,"given":"Daniel J.","family":"Wilkinson","sequence":"additional","affiliation":[{"name":"MRC\u2010Versus Arthritis Centre for Musculoskeletal Ageing Research, National Institute for Health Research Nottingham Biomedical Research Centre University of Nottingham  Nottingham UK"},{"name":"Division of Health Sciences and Graduate Entry Medicine School of Medicine Royal Derby Hospital Centre  Derby UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,22]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01069.2014"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2010.29819"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.225003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01069.2014"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.TIR119.001906"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-009-1136-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glu313"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000413"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000652"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000392"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP272857"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-273755"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12532"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00481.2014"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012033"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201700531R"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.228833"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-2640fje"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu11071657"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.135392"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.142828"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00230.2016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2018.09.025"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075197-200309000-00004"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-anchem-071114-040325"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv271"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70218-7"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12877-017-0512-6"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1989.257.6.E828"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12472"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90411.2008"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2015.05.018"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2018.07.018"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00488.2005"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-018-4673-2"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.164483"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-2004"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac991131p"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.093690"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glz113"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2011.00769.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00611.2012"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr060599n"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20171230"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.115.011650"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2012.00260"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2017-097608"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.136424"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP275246"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx009"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqz332"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2018.12621"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2018.1511918"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200202)2:2<157::AID-PROT157>3.0.CO;2-M"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0630519"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15364"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.8b00417"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI79639"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00257.2015"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1992.262.3.E372"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1998.275.1.E73"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.28256"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00076.2009"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3553"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2018.10.022"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12029"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201400038"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.13.5892"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.14336\/AD.2017.0315"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.27543"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21507"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP272277"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2017.09.008"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12433"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00650.2013"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2018.07.005"},{"key":"e_1_2_7_78_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.045708"},{"key":"e_1_2_7_79_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.055517"},{"key":"e_1_2_7_80_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.3.475"},{"key":"e_1_2_7_81_1","volume-title":"Isotope tracers in metabolic research: Principles and practice of kinetic analysis","author":"Wolfe R. R.","year":"2005"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP087492","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP087492","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP087492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T11:29:37Z","timestamp":1693913377000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP087492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,22]]},"references-count":80,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,7]]}},"alternative-id":["10.1113\/EP087492"],"URL":"https:\/\/doi.org\/10.1113\/ep087492","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,22]]},"assertion":[{"value":"2020-03-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-05-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}