{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:10:28Z","timestamp":1747188628758,"version":"3.40.5"},"reference-count":65,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,1,19]],"date-time":"2020-01-19T00:00:00Z","timestamp":1579392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004795","name":"Institut Universitaire de France","doi-asserted-by":"publisher","award":["IUF"],"award-info":[{"award-number":["IUF"]}],"id":[{"id":"10.13039\/501100004795","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>What are the effects of repeated subclinical vaso\u2010occlusions on nuclear factor erythroid 2 related factor 2 (Nrf2) and oxidative stress balance regulation in the kidney of transgenic SAD mice?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>In response to hypoxia\u2013reoxygenation, nuclear Nrf2 protein expression decreased in the kidney of SAD mice while haem oxygenase transcripts were increased. This suggest that in SAD mice, other transcription factors than Nrf2 could be involved in renal antioxidant gene regulation in response to hypoxia\u2013reoxygenation.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Hypoxia\u2013reoxygenation (H\/R) stress is known to increase oxidative stress in transgenic sickle mice and can cause organ failure. Here we described the effects of H\/R on nuclear factor erythroid 2\u2010related factor 2 (Nrf2) as a putative regulator of redox status in the kidneys of SAD mice investigating Nrf2\u2010regulated antioxidant enzymes. Transgenic SAD mice and healthy C57Bl\/6J mice were exposed to 4\u00a0h of hypoxia followed by various times of reoxygenation at ambient air (2 or 6\u00a0h). Regardless of the conditions (i.e. normoxia or H\/R), SAD mice expressed higher renal oxidative stress levels. Nuclear Nrf2 protein expression decreased after 2\u00a0h post\u2010hypoxia only in the medulla region of the kidney and only in SAD mice. Simultaneously, haem oxygenase transcripts were affected by H\/R stimulus with a significant enhancement after 2\u00a0h post\u2010hypoxia. Similarly, hypoxia inducible factor\u20101\u03b1 staining increased after 2\u00a0h post\u2010hypoxia in SAD mice in both cortex and medulla areas. Our data confirm that the kidneys are organs that are particularly sensitive to H\/R stimuli in sickle cell SAD mice. Also, these results suggest an effect of the duration of recovery period (short <jats:italic>vs<\/jats:italic>. long) and specific responses according to kidney areas, medulla <jats:italic>vs<\/jats:italic>. cortex, on Nrf2 expression in response to H\/R stimuli in SAD mice.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep087969","type":"journal-article","created":{"date-parts":[[2019,12,6]],"date-time":"2019-12-06T00:53:41Z","timestamp":1575593621000},"page":"357-369","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of hypoxia\u2013reoxygenation stimuli on renal redox status and nuclear factor erythroid 2\u2010related factor 2 pathway in sickle cell SAD mice"],"prefix":"10.1113","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7047-2482","authenticated-orcid":false,"given":"Camille","family":"Faes","sequence":"first","affiliation":[{"name":"Interuniversity Laboratory of Human Movement Biology 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