{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:10:30Z","timestamp":1747188630525,"version":"3.40.5"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2019,10,24]],"date-time":"2019-10-24T00:00:00Z","timestamp":1571875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["307019\/2013\u20106"],"award-info":[{"award-number":["307019\/2013\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004586","name":"Funda\u00e7\u00e3o Carlos Chagas Filho de Amparo \u00e0 Pesquisa do Estado do Rio de Janeiro","doi-asserted-by":"publisher","award":["proc. nos E26\/110.045\/2014","E26\/202.981\/2015"],"award-info":[{"award-number":["proc. nos E26\/110.045\/2014","E26\/202.981\/2015"]}],"id":[{"id":"10.13039\/501100004586","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>Can Na<jats:sup>+<\/jats:sup> depletion mobilize Na<jats:sup>+<\/jats:sup> from the skin reservoir in ovariectomized rats? Does oestrogen replacement change the amount and the dynamics of skin Na<jats:sup>+<\/jats:sup> storage? Is the reduced salt appetite after Na<jats:sup>+<\/jats:sup> depletion in ovariectomized rats with oestrogen replacement related to changes in the skin Na<jats:sup>+<\/jats:sup>?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>This work demonstrated that acute body Na<jats:sup>+<\/jats:sup> depletion induced by frusemide mobilized the osmotically inactive skin Na<jats:sup>+<\/jats:sup> reservoir to become osmotically active. Oestrogen treatment decreased the induced Na<jats:sup>+<\/jats:sup> intake in ovariectomized rats but did not modulate the inactive Na<jats:sup>+<\/jats:sup> reservoir in control conditions or its mobilization induced by Na<jats:sup>+<\/jats:sup> depletion.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Oestradiol, which is an important hormone for water and electrolyte balance, also has a role in the inhibition of induced Na<jats:sup>+<\/jats:sup> appetite. Sodium can be stored in the skin in osmotically active or inactive forms, and this skin Na<jats:sup>+<\/jats:sup> reservoir may be involved in the control of body Na<jats:sup>+<\/jats:sup> levels during physiopathological challenges. In this study, we investigated whether the effect of sodium depletion by frusemide can mobilize Na<jats:sup>+<\/jats:sup> from the skin reservoir and whether oestradiol replacement changes or mobilizes the Na<jats:sup>+<\/jats:sup> reserves in the skin. Ovariectomized Wistar rats were treated with vehicle or oestradiol for 7\u00a0days to evaluate the effects of oestrogen on the hydroelectrolyte balance, intake responses and skin Na<jats:sup>+<\/jats:sup> and water content in basal conditions. Furthermore, the effects of oestrogen were evaluated after 24 h frusemide\u2010induced whole\u2010body Na<jats:sup>+<\/jats:sup> depletion. Oestradiol\u2010replaced rats exhibited reduced water intake without any significant changes in salt intake, Na<jats:sup>+<\/jats:sup> excretion or water and Na<jats:sup>+<\/jats:sup> skin content in basal conditions. After sodium depletion, both vehicle\u2010 and oestradiol\u2010treated rats exhibited an increase in the osmotically active skin Na<jats:sup>+<\/jats:sup>, which was associated with a decrease of the inactive skin Na<jats:sup>+<\/jats:sup> reservoir. Oestrogen decreased the hypertonic saline intake induced by Na<jats:sup>+<\/jats:sup> depletion, but it was not associated with any significant changes in the skin Na<jats:sup>+<\/jats:sup> reservoir. Thus, sodium depletion is able to change the inactive\u2013active skin Na<jats:sup>+<\/jats:sup> reservoir balance. However, the oestrogenic modulation of sodium appetite after Na<jats:sup>+<\/jats:sup> depletion is probably not related to the action of this hormone in the skin Na<jats:sup>+<\/jats:sup> reservoir balance.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep087998","type":"journal-article","created":{"date-parts":[[2019,9,25]],"date-time":"2019-09-25T20:41:18Z","timestamp":1569444078000},"page":"1754-1761","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Acute body sodium depletion induces skin sodium mobilization in female Wistar rats"],"prefix":"10.1113","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9624-9182","authenticated-orcid":false,"given":"V. 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