{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:40Z","timestamp":1747188460070,"version":"3.40.5"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T00:00:00Z","timestamp":1600905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"publisher","award":["2016YFC1304403","2016YFC0903700"],"award-info":[{"award-number":["2016YFC1304403","2016YFC0903700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81560013","81520108001","81700043"],"award-info":[{"award-number":["81560013","81520108001","81700043"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>What is the role of breast cancer type 1 interacting protein C\u2010terminal helicase 1 (<jats:italic>BRIP1<\/jats:italic>) polymorphism in chronic obstructive pulmonary disease (COPD)?<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Variant rs10744996C&gt;A of <jats:italic>BRIP1<\/jats:italic> increases the susceptibility of the Mongolian population to COPD. The expression of <jats:italic>BRIP1<\/jats:italic> was significantly reduced in cigarette smoke extract\u2010treated airway epithelial cells.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Cigarette smoke is a major environmental pollutant that can induce DNA damage in humans. The development and progression of chronic obstructive pulmonary disease (COPD) are known to be related to the impairment of DNA repair. Breast cancer type 1 interacting protein C\u2010terminal helicase 1 (BRIP1) plays an important role in DNA interstrand crosslink repair and double\u2010strand break repair. However, the role of <jats:italic>BRIP1<\/jats:italic> polymorphisms in COPD has not been previously described. In this study, whole genome sequencing was used to identify mutations, and single nucleotide polymorphism (SNP) genotyping was used to verify the selected SNPs. In addition the <jats:italic>BRIP1<\/jats:italic> expression levels in 16HBE and A549 airway epithelial cells treated with or without cigarette smoke extract (CSE) were measured using western blotting and RT\u2010qPCR. Rs10744996C&gt;A in the 3\u2032\u2010untranslated region (3\u2032UTR) of <jats:italic>BRIP1<\/jats:italic> was then genotyped in 1296 COPD cases and 988 healthy control subjects from a Mongolian population in northern China. Significant differences in the distribution of rs10744996C&gt;A variants between COPD and control groups (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.001) were identified. Rs10744996C&gt;A was found to be associated with significantly increased COPD risk (adjusted odds ratio\u00a0=\u00a01.60, 95% CI\u00a0=\u00a01.30\u20131.98, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.0001). Additionally, rs10744996A genotype was found to interact with a family history of cancer and a history of x\u2010ray exposure (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.028 and 0.009, respectively). <jats:italic>BRIP1<\/jats:italic> expression levels in 16HBE and A549 cells treated with CSE were significantly lower compared to the control treated cells. The rs10744996C&gt;A variant of <jats:italic>BRIP1<\/jats:italic> increased the COPD susceptibility of the Mongolian population cohort. BRIP1 mRNA and protein expression levels were significantly reduced in conjunction with CSE\u2010induced DNA damage in 16HBE and A549 cells.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep088210","type":"journal-article","created":{"date-parts":[[2020,8,27]],"date-time":"2020-08-27T12:28:41Z","timestamp":1598531321000},"page":"1950-1959","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<i>BRIP1<\/i> rs10744996C&gt;A variant increases the risk of chronic obstructive pulmonary disease in the Mongolian population of northern China"],"prefix":"10.1113","volume":"105","author":[{"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Renmin Hospital of Wuhan University  Wuhan Hubei China"},{"name":"Department of Rehabilitation Medicine Affiliated Hospital of Inner Mongolia Medical University  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dejun","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine Inner Mongolia Autonomous Region People's Hospital  Hohhot Inner Mongolia China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenju","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institute of Respiratory Health The First Affiliated Hospital of Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zili","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institute of Respiratory Health The First Affiliated Hospital of Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenting","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Respiratory Disease Guangzhou Institute of Respiratory Health The First Affiliated Hospital of Guangzhou Medical University  Guangzhou Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9862-7239","authenticated-orcid":false,"given":"Ke","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Respiratory and Critical Care Medicine Renmin Hospital of Wuhan University  Wuhan Hubei 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