{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T14:24:09Z","timestamp":1783779849554,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,3,29]],"date-time":"2020-03-29T00:00:00Z","timestamp":1585440000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2020,5]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of the study?<\/jats:bold><\/jats:p>\n<jats:p>Does \u03b2\u2010alanine with <jats:sc>l<\/jats:sc>\u2010histidine supplementation associated with endurance and strength training improve echocardiographic parameters, functional capacity, and maximum strength in rats with chronic heart failure?<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>\u03b2\u2010Alanine with <jats:sc>l<\/jats:sc>\u2010histidine supplementation associated with endurance and strength training increased functional capacity and maximum strength through increasing exercise capacity peripherally but did not affect echocardiographic parameters in rats with chronic heart failure.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:label\/><jats:p>Combined training (CT) has been associated with positive responses in the clinical status of patients with chronic heart failure (CHF). Other non\u2010pharmacological tools, such as amino acid supplementation, may further enhance its adaptation. However, the effects of \u03b2\u2010alanine and <jats:sc>l<\/jats:sc>\u2010histidine supplementation in CHF remain unclear. In the present study, the aim was to test whether supplementing carnosine precursors with CT could give improved responses in the functional capacity and echocardiographic variables of rats with CHF. Twenty\u2010four Wistar rats, were submitted to myocardial infarction and allocated to three groups: animals with CHF kept in sedentary conditions (SED, <jats:italic>n<\/jats:italic>\u00a0=\u00a08), animals with CHF submitted to CT in strength and aerobic exercise supplemented with placebo (CT\u2010P, <jats:italic>n<\/jats:italic>\u00a0=\u00a08) and animals with CHF submitted to CT in strength and aerobic exercise supplemented with \u03b2\u2010alanine and <jats:sc>l<\/jats:sc>\u2010histidine (CT\u2010S, <jats:italic>n<\/jats:italic>\u00a0=\u00a08). The trained animals were submitted to a strength protocol three times per week with intensity of 65\u201375% of one repetition maximum test. Aerobic training was conducted two times per week (50\u00a0min, 15\u00a0m\u00a0min<jats:sup>\u22121<\/jats:sup>). The supplemented group received \u03b2\u2010alanine and <jats:sc>l<\/jats:sc>\u2010histidine orally (each 250\u00a0mg\u00a0kg<jats:sup>\u22121<\/jats:sup>\u00a0day<jats:sup>\u22121<\/jats:sup>). No changes in echocardiographic and morphological parameters were found among the groups (<jats:italic>P\u00a0<\/jats:italic>&gt;\u00a00.05). Functional capacity, \u0394<jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/eph12710-math-0001.png\" xlink:title=\"urn:x-wiley:09580670:media:eph12710:eph12710-math-0001\"\/> and maximum strength were higher in CT\u2010P than in SED and even higher in CT\u2010S than in CT\u2010P (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.01). The CT was able to improve functional capacity, but the supplementation was shown to enhance these parameters even further in the CHF rats. We conclude that the increase in functional capacity and strength gained through CT and supplementation were associated with the improvement in peripheral parameters with no changes in cardiac variables.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep088327","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T14:34:12Z","timestamp":1583246052000},"page":"831-841","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["\u03b2\u2010Alanine and <scp>l<\/scp>\u2010histidine supplementation associated with combined training increased functional capacity and maximum strength in heart failure rats"],"prefix":"10.1113","volume":"105","author":[{"given":"Giuseppe Potrick","family":"Stefani","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Physiology, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  Porto Alegre RS Brazil"},{"name":"Graduate Program in Rehabilitation Sciences, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas","family":"Capalonga","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiology, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas Ribeiro","family":"da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiology, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  Porto Alegre RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9907-7689","authenticated-orcid":false,"given":"Pedro","family":"Dal Lago","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Physiology, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  Porto Alegre RS Brazil"},{"name":"Graduate Program in Rehabilitation Sciences, Universidade Federal de Ci\u00eancias da Sa\u00fade de Porto Alegre (UFCSPA)  RS Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,3,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardfail.2015.10.004"},{"key":"e_1_2_9_3_1","first-page":"5432","article-title":"High\u2010intensity resistance training alone or combined with aerobic training improves strength, heart function and collagen in rats with heart failure","volume":"9","author":"Alves J. P.","year":"2017","journal-title":"American Journal of Translational Research"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110317"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2019.03.009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-018-2593-8"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2016-0095"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00039.2012"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00019.2016"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10103-016-1922-y"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1381612823666170124120518"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2165\/11530310-000000000-00000"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hfc.2017.02.001"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e31826cdb68"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharep.2013.08.008"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.110.168252"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP085299"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP086076"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2018.11.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2017-0069"},{"key":"e_1_2_9_21_1","volume-title":"Resource Book for the Design of Animal Exercise Protocols","author":"Kregel K. C. A. D.","year":"2006"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327115597468"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.echo.2005.10.005"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1993.1017"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nut.2014.04.021"},{"key":"e_1_2_9_26_1","first-page":"511","article-title":"Neuroprotective effect of carnosine on primary culture of rat cerebellar cells under oxidative stress","volume":"81","author":"Lopachev A. V.","year":"2016","journal-title":"Biochemistry"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1136\/openhrt-2014-000119"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/19390211.2016.1244585"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1590\/bjpt-rbf.2014.0098"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1519\/JSC.0000000000000665"},{"issue":"7","key":"e_1_2_9_31_1","first-page":"547","article-title":"2016 European guidelines on cardiovascular disease prevention in clinical practice: The Sixth Joint Task Force of the European Society of Cardiology and Other Societies on Cardiovascular Disease Prevention in Clinical Practice (constituted by representatives of 10 societies and by invited experts). Developed with the special contribution of the European Association for Cardiovascular Prevention & Rehabilitation (EACPR)","volume":"18","author":"Piepoli M. F.","year":"2017","journal-title":"Giornale Italiano Di Cardiologia"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cnur.2015.07.012"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2015-0391"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2016-096396"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0204858"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1249\/00005768-199208000-00009"},{"issue":"1","key":"e_1_2_9_37_1","first-page":"187","article-title":"Pathophysiology of heart failure","volume":"6","author":"Tanai E.","year":"2016","journal-title":"Comprehensive Physiology"},{"issue":"4","key":"e_1_2_9_38_1","first-page":"CD003331.","article-title":"Exercise\u2010based rehabilitation for heart failure","author":"Taylor R. S.","year":"2014","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.12.072"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.13938"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2018.1475269"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/nhs.12593"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529115"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCHEARTFAILURE.117.004129"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1997.272.1.H462"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2FEP088327","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP088327","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP088327","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP088327","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T21:53:04Z","timestamp":1694037184000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP088327"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,3,29]]},"references-count":44,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5]]}},"alternative-id":["10.1113\/EP088327"],"URL":"https:\/\/doi.org\/10.1113\/ep088327","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,29]]},"assertion":[{"value":"2019-11-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}