{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:07:57Z","timestamp":1747188477046,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,11]],"date-time":"2020-08-11T00:00:00Z","timestamp":1597104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item>\n<jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>The aim of the experiment was to highlight the regenerative capacity of bone marrow Kit<jats:sup>+<\/jats:sup> cells in the restoration of asthmatic pulmonary function in the rat model.<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Data showed that these cells were recruited successfully to the asthmatic niche after intratracheal administration and accelerated the regeneration of asthmatic lungs by the modulation of inflammation via the control of <jats:italic>Gata3<\/jats:italic> and <jats:italic>Tbx21<\/jats:italic> expression, leading to decreased tracheal responsiveness to methacholine and reduction of pathological remodelling.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Allergic asthma is a T\u00a0helper (Th)\u00a02 immunological disorder with consequential uncontrolled inflammatory responses. There is an increasing demand to use new methods for the treatment of asthma based on modulation of the Th2\u2010to\u2010Th1 ratio in favour of the Th1 population. Accordingly, we decided to evaluate the effects of intratracheal administration of Kit<jats:sup>+<\/jats:sup> bone marrow cells on tracheal responsiveness and the expression of <jats:italic>Gata3<\/jats:italic> and <jats:italic>Tbx21<\/jats:italic> genes. Forty male Wistar rats were allocated\u00a0 randomly\u00a0 into four experimental groups: healthy rats (control group), sensitized rats (OVA group), sensitized rats receiving Kit<jats:sup>\u2212<\/jats:sup> cells (OVA+Kit<jats:sup>\u2212<\/jats:sup> group) and sensitized rats receiving Kit<jats:sup>+<\/jats:sup> cells (OVA+Kit<jats:sup>+<\/jats:sup> group). Total and differential white blood cell counts, tracheal responsiveness to cumulative methacholine concentrations and histopathological analysis were evaluated. The results showed a statistically significant increase in total white blood cell, eosinophil and neutrophil counts, tracheal contractility, <jats:italic>Gata3<\/jats:italic> expression and prototypical histopathology of asthma. Along with these conditions, we found that the number of lymphocytes was decreased and expression of <jats:italic>Tbx21<\/jats:italic> diminished in sensitized rats compared with control animals. Monitoring of labelled tagged cells confirmed successful engraftment of transplanted cells in pulmonary tissue. Juxtaposition of Kit<jats:sup>+<\/jats:sup> cells changed the blood leucogram closer to the control values. Kit<jats:sup>+<\/jats:sup> cells increased the expression of <jats:italic>Tbx21<\/jats:italic> and suppressed <jats:italic>Gata3<\/jats:italic> (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). In the OVA+Kit<jats:sup>+<\/jats:sup> group, tracheal responsiveness was improved coincident with increased pulmonary regeneration. In conclusion, this study showed that intratracheal\u00a0administration of bone marrow\u2010derived Kit<jats:sup>+<\/jats:sup> cells, but not Kit<jats:sup>\u2212<\/jats:sup> cells, could be effective in the alleviation of asthma, presumably by the modulation of <jats:italic>Gata3<\/jats:italic> and <jats:italic>Tbx21<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep088633","type":"journal-article","created":{"date-parts":[[2020,7,27]],"date-time":"2020-07-27T08:15:52Z","timestamp":1595837752000},"page":"1623-1633","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["C\u2010Kit<sup>+<\/sup> progenitors restore rat asthmatic lung function by modulation of T\u2010bet and GATA\u20103 expression"],"prefix":"10.1113","volume":"105","author":[{"given":"Hossein","family":"Heiran","sequence":"first","affiliation":[{"name":"Department of Physiology Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdi","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Tuberculosis and Lung Dsiseases Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Rahbarghazi","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Applied Cell Sciences Faculty of Advanced Medical Sciences Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatemeh","family":"Mir\u2010ershadi","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Physiology Ardabil Branch Islamic Azad University  Ardabil Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aref","family":"Delkhosh","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Pathobiology Faculty of Veterinary Medicine Urmia University  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Khaksar","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morteza","family":"Heidarzadeh","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6941-2690","authenticated-orcid":false,"given":"Rana","family":"Keyhanmanesh","sequence":"additional","affiliation":[{"name":"Department of Physiology Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Drug Applied Research Center Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Neurogenic Inflammation Research Center Mashhad University of Medical Sciences  Mashhad Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01675-x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.11.127"},{"issue":"3","key":"e_1_2_9_4_1","doi-asserted-by":"crossref","first-page":"347","DOI":"10.56808\/2985-1130.2922","article-title":"Monitoring IL\u201013 expression in relation to miRNA\u2010155 and miRNA\u2010133 changes following intra\u2010tracheal administration of mesenchymal stem cells and conditioned media in ovalbuminsensitized rats","volume":"48","author":"Ahmadi M.","year":"2018","journal-title":"The Thai Journal of Veterinary Medicine"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.4149\/gpb_2017052"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0431-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2020.117446"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1465-9921-12-114"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.10.005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/726740"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI27019"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0751"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2018.64.10.15"},{"key":"e_1_2_9_14_1","first-page":"255","article-title":"The effect of alpha linolenic acid on tracheal responsiveness, lung inflammation, and immune markers in sensitized rats","volume":"22","author":"Kaveh M.","year":"2019","journal-title":"Iranian journal of basic medical sciences"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0866-8"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2018.09.049"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbf.3354"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201801710"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.02.002"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2018.10.005"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.28480"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.21037\/sci.2019.06.04"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3727\/096368913X667736"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-2098-9_7"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3917471"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0158746"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140798"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/2050-490X-2-7"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2FEP088633","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP088633","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP088633","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP088633","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T12:12:13Z","timestamp":1693915933000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP088633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,11]]},"references-count":27,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1113\/EP088633"],"URL":"https:\/\/doi.org\/10.1113\/ep088633","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"type":"print","value":"0958-0670"},{"type":"electronic","value":"1469-445X"}],"subject":[],"published":{"date-parts":[[2020,8,11]]},"assertion":[{"value":"2020-03-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}