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During heat stress, core and mean skin temperatures, vasodilatory responses, sweat loss, heart rate and left ventricular function were unchanged, and mean arterial pressure was only transiently reduced, post\u2010supplementation. These data suggest nitrate supplementation with beetroot juice does not mitigate thermoregulatory or cardiovascular strain in heat\u2010stressed older individuals.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>This study tested the hypothesis that dietary nitrate supplementation with concentrated beetroot juice attenuates thermoregulatory and cardiovascular strain in older individuals during environmental heat stress. Nine healthy older individuals (six females, three males; aged 67\u00a0\u00b1\u00a05\u00a0years) were exposed to 42.5\u00a0\u00b1\u00a00.1\u00b0C and 34.0\u00a0\u00b1\u00a00.5% relative humidity conditions for 120\u00a0min before (CON) and after 7\u00a0days of dietary nitrate supplementation with concentrated beetroot juice (BRJ; 280\u00a0ml, \u223c16.8\u00a0mmol of nitrate daily). Core and skin temperatures, body mass changes (indicative of whole\u2010body sweat loss), skin blood flow and cutaneous vascular conductance, forearm blood flow and vascular conductance, heart rate, arterial blood pressures and indices of cardiac function were measured. The 7\u2010day beetroot juice regimen increased plasma nitrate\/nitrite levels from 27.4\u00a0\u00b1\u00a015.2 to 477.0\u00a0\u00b1\u00a0102.5\u00a0\u03bcmol\u00a0l<jats:sup>\u22121<\/jats:sup> (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01) and lowered resting mean arterial pressure from 90\u00a0\u00b1\u00a07 to 83\u00a0\u00b1\u00a010\u00a0mmHg at baseline under thermoneutral conditions (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.02). However, during subsequent heat stress, no differences in core and skin temperatures, skin blood flow and vascular conductance, forearm blood flow and vascular conductance, whole\u2010body sweat loss, heart rate, and echocardiographic indices of systolic function and diastolic filling were evident following nitrate supplementation (all <jats:italic>P<\/jats:italic>\u00a0&gt;\u00a00.05). Mean arterial pressure was lower in BRJ <jats:italic>vs<\/jats:italic>. CON during heat stress (treatment\u2010by\u2010time interaction: <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02). Overall, these findings suggest that dietary nitrate supplementation with concentrated beetroot juice does not attenuate thermoregulatory or cardiovascular strain in older individuals exposed to severe ambient heat stress.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep088834","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T01:32:59Z","timestamp":1598578379000},"page":"1730-1741","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dietary nitrate supplementation does not influence thermoregulatory or cardiovascular strain in older individuals during severe ambient heat stress"],"prefix":"10.1113","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7400-8353","authenticated-orcid":false,"given":"Matthew N.","family":"Cramer","sequence":"first","affiliation":[{"name":"Institute for Exercise and Environmental Medicine Texas Health Presbyterian Hospital Dallas and University of Texas Southwestern Medical Center  Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michinari","family":"Hieda","sequence":"additional","affiliation":[{"name":"Institute for Exercise and Environmental Medicine Texas Health Presbyterian Hospital Dallas and University of Texas Southwestern Medical Center  Dallas TX USA"},{"name":"School of Medicine Kyushu University  Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mu","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute for Exercise and Environmental Medicine Texas Health Presbyterian Hospital Dallas and University of Texas Southwestern Medical Center  Dallas TX USA"},{"name":"Applied Clinical Research University of Texas Southwestern Medical Center  Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0654-2383","authenticated-orcid":false,"given":"Gilbert","family":"Moralez","sequence":"additional","affiliation":[{"name":"Institute for Exercise and Environmental Medicine Texas Health Presbyterian Hospital Dallas and University of Texas Southwestern Medical Center  Dallas TX USA"},{"name":"Applied Clinical Research University of Texas Southwestern Medical Center  Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7792-9713","authenticated-orcid":false,"given":"Craig G.","family":"Crandall","sequence":"additional","affiliation":[{"name":"Institute for Exercise and Environmental Medicine Texas Health Presbyterian Hospital Dallas and University of Texas Southwestern Medical Center  Dallas TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,31]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00421-018-3889-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1532-5415.1981.tb01238.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0278-6915(84)90116-9"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/368502a0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01069.2008"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/sms.12848"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.239566"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00786.2019"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1974.37.2.247"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0695-546"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1957.sp005904"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00420-006-0089-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/17461391.2020.1739144"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00714.2015"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP087049"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2008.08.003"},{"key":"e_1_2_9_18_1","first-page":"273","article-title":"Further observations on the vascular responses of the human limb to body warming; evidence for sympathetic vasodilator nerves in the normal subject","volume":"3","author":"Grant R. 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