{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:16:05Z","timestamp":1775175365302,"version":"3.50.1"},"reference-count":65,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,4,13]],"date-time":"2021-04-13T00:00:00Z","timestamp":1618272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item>\n<jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>In adult rat hearts, exposure to hypobaric hypoxia increases tolerance to hypoxia\u2013reoxygenation, termed endogenous cardioprotection. The mechanism involves the nitric oxide system and modulation of mitochondrial oxygen consumption. What is the cardiac energetic response in prepubertal rats exposed to hypobaric hypoxia?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Prepubertal rats, unlike adult rats, did not increase tolerance to hypoxia\u2013reoxygenation in response acute exposure to hypobaric hypoxia, which impaired cardiac contractile economy. This finding could be related to a failure to increase nitric oxide synthase expression, hence modulation of mitochondrial oxygen consumption and ATP production.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Studies in our laboratory showed that exposure of rats to hypobaric hypoxia (HH) increased the tolerance of the heart to hypoxia\u2013reoxygenation (H\/R), involving mitochondrial and cytosolic nitric oxide synthase (NOS) systems. The objective of the present study was to evaluate how the degree of somatic maturation could alter this healthy response. Prepubertal male rats were exposed for 48\u00a0h to a simulated altitude of 4400\u00a0m in a hypobaric chamber. The mechanical energetic activity in perfused hearts and the contractile functional capacity of NOS in isolated left ventricular papillary muscles were evaluated during H\/R. Cytosolic nitric oxide (NO), production of nitrites\/nitrates (Nx), expression of NOS isoforms, mitochondrial O<jats:sub>2<\/jats:sub> consumption and ATP production were also evaluated. The left ventricular pressure during H\/R was not improved by HH. However, the energetic activity was increased. Thus, the contractile economy (left ventricular pressure\/energetic activity) decreased in HH. Nitric oxide did not modify papillary muscle contractility after H\/R. Cytosolic p\u2010eNOS\u2010Ser1177 and inducible NOS expression were decreased by HH, but no changes were observed in NO production. Interestingly, HH increased Nx levels, but O<jats:sub>2<\/jats:sub>\u00a0consumption and ATP production in mitochondria were not affected\u00a0by HH. Prepubertal rats exposed to HH preserved cardiac contractile function, but with a high energetic cost, modifying contractile economy. Although this\u00a0could be related to the decreased NOS expression detected, cytosolic NO production was preserved, maybe through the Nx metabolic pathway, without modification of mitochondrial ATP production and O<jats:sub>2<\/jats:sub>\u00a0consumption. In this scenario, the treatment was unable to increase tolerance to H\/R as observed in adult animals.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep089064","type":"journal-article","created":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T12:42:47Z","timestamp":1615898567000},"page":"1235-1248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Acute hypobaric hypoxia and cardiac energetic response in prepubertal rats: Role of nitric oxide"],"prefix":"10.1113","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2263-4648","authenticated-orcid":false,"given":"Pablo H.","family":"La Padula","sequence":"first","affiliation":[{"name":"Facultad de Medicina Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional (IATIMET), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Analia","family":"Czerniczyniec","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica Instituto de Bioqu\u00edmica y Medicina Molecular (IBIMOL), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia","family":"Bonazzola","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional (IATIMET), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B\u00e1rbara","family":"Piotrkowski","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica Instituto de Bioqu\u00edmica y Medicina Molecular (IBIMOL), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia","family":"Vanasco","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica Instituto de Bioqu\u00edmica y Medicina Molecular (IBIMOL), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Lores\u2010Arnaiz","sequence":"additional","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica Instituto de Bioqu\u00edmica y Medicina Molecular (IBIMOL), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lidia E.","family":"Costa","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Instituto Alberto C. Taquini de Investigaciones en Medicina Traslacional (IATIMET), Universidad de Buenos Aires (UBA) \u2013 Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET)  Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/152308604322899495"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb09239.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6363(99)00163-7"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1748-1716.2010.02094.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)01082-4"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)59196-5"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0531-5565(00)00135-2"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2007.03.022"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0178"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2004.42"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00526.2005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.883533"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1139\/Y08-095"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00321"},{"key":"e_1_2_8_16_1","first-page":"153","volume-title":"Advances in Chemistry and Biology of Nitric Oxide","author":"Costa L. E","year":"2007"},{"key":"e_1_2_8_17_1","unstructured":"Costa L. E. &La PadulaP.(2019).Mitochondrial response during exposure to hypobaric hypoxia. In Brain mitochondria: Distribution and function. Chapter 7 (pp.239\u2013276).Nova Science Publishers Series: Neuroscience research progress cell biology research progress. ISBN: 978\u20101\u201053616\u2010650\u20107."},{"key":"e_1_2_8_18_1","first-page":"604","article-title":"Effect of chronic hypobaric hypoxia on the growth and body composition of the rat","volume":"39","author":"Costa L. E.","year":"1979","journal-title":"Medicina"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200604016"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.appet.2016.07.005"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcmd.2004.02.002"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00264.2003"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12376"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(67)10010-4"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-009-0767-7"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio1005-245"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00407.2006"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio1105-308"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.01164.2010"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2006.033506"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2828(03)00143-3"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.54.070185.005055"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2010.01.004"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.92"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.120.019240"},{"key":"e_1_2_8_36_1","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.33549\/physiolres.930000.53.S3","article-title":"Molecular mechanisms of cardiac protection by adaptation to chronic hypoxia","volume":"53","author":"Kol\u00e1\u0159 F.","year":"2004","journal-title":"Physiological Research"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.90400.2008"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00988.2004"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2017.12.007"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000161048.72004.c2"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1159\/000363001"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020623100401"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/niox.2000.0319"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchemneu.2012.12.003"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2010.03.010"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.10.003"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(94)33027-1"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200210000-00016"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0653"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00534.2002"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1996.0146"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.53.37709"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00374809"},{"key":"e_1_2_8_54_1","first-page":"445","article-title":"Energetics of ionic behavior in heart muscle contraction. Physiologic and physiopathologic aspects","volume":"53","author":"Ponce\u2010Hornos J. E.","year":"1993","journal-title":"Medicina"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-005-5451-2"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2007.07.019"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1095-6433(98)01010-1"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00001.2004"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00770.2003"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2012.12.002"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00408635"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6363(96)00083-1"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00920.2001"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5348.234"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00986.2004"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9226"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP089064","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP089064","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP089064","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T08:48:42Z","timestamp":1693558122000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP089064"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,13]]},"references-count":65,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,5]]}},"alternative-id":["10.1113\/EP089064"],"URL":"https:\/\/doi.org\/10.1113\/ep089064","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,13]]},"assertion":[{"value":"2020-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-03-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}