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They displayed highly dysmorphic mitochondria and mitophagy in skeletal muscle.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Cachexia causes changes in skeletal muscle metabolism. Mice with MDA\u2010MB\u2010231 breast cancer bone metastases and cachexia have decreased whole animal energy metabolism and increased skeletal muscle mitophagy. We examined whole animal energy metabolism by indirect calorimetry in mice with MDA\u2010MB\u2010231 breast cancer bone metastases, and showed decreased energy expenditure. We also examined skeletal muscle mitochondria and found that mitochondria in mice with MDA\u2010MB\u2010231 bone metastases are highly dysmorphic and have altered protein markers of mitochondrial biogenesis and dynamics. In addition, LC3B protein was increased in mitochondria of skeletal muscle from cachectic mice, and colocalized with the mitochondrial protein Tom20. Our data demonstrate the importance of mitophagy in cachexia. Understanding these changes will help contribute to defining treatments for cancer cachexia.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep089130","type":"journal-article","created":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T20:58:32Z","timestamp":1609189112000},"page":"506-518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Bone metastases induce metabolic changes and mitophagy in mice"],"prefix":"10.1113","volume":"106","author":[{"given":"Jenna","family":"Wilcox\u2010Hagerty","sequence":"first","affiliation":[{"name":"The Penn State College of Medicine Department of Cellular and Molecular Physiology  Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifang","family":"Xu","sequence":"additional","affiliation":[{"name":"The Penn State College of Medicine Department of Cellular and Molecular Physiology  Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian A.","family":"Hain","sequence":"additional","affiliation":[{"name":"The Penn State College of Medicine Department of Cellular and Molecular Physiology  Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy C.","family":"Arnold","sequence":"additional","affiliation":[{"name":"The Penn State College of Medicine Department of Neural and Behavioral Sciences  Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3858-7623","authenticated-orcid":false,"given":"David L.","family":"Waning","sequence":"additional","affiliation":[{"name":"The Penn State College of Medicine Department of Cellular and Molecular Physiology  Hershey PA USA"},{"name":"Penn State Cancer Institute  Hershey PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,1,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101660"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2016.04.019"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30340"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.14033"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"472","DOI":"10.3389\/fphys.2016.00472","article-title":"Cancer and chemotherapy contribute to muscle loss by activating common signaling pathways","volume":"7","author":"Barreto R.","year":"2016","journal-title":"Frontiers in Physiology"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9779"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.06.043"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00004.2014"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-1723"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00039.2012"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/bonekey.2015.101"},{"issue":"6","key":"e_1_2_8_13_1","first-page":"1087","article-title":"Increased mitophagy in the skeletal muscle of spinal and bulbar muscular atrophy patients","volume":"26","author":"Borgia D.","year":"2017","journal-title":"Human Molecular Genetics"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12232"},{"key":"e_1_2_8_15_1","first-page":"98","volume-title":"Sabiston Textook of Surgery","author":"Carson J.","year":"2017"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.24611"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.07.040"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-11327-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407043101"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1987.13"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4081\/ejtm.2018.7587"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.08.061"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpainsymman.2006.09.024"},{"issue":"5","key":"e_1_2_8_24_1","first-page":"158","article-title":"Chemotherapy\u2010induced muscle wasting: Assocation with NF\u2010\u03baB and cancer cachexia","volume":"18","author":"Damrauer J. 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