{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:16:16Z","timestamp":1791263776307,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T00:00:00Z","timestamp":1626912000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item>\n<jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>Is lymphocyte DNA methylation differentially modulated by resistance training and aerobic exercise in older women?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>The practice of resistance training led to an increased global DNA methylation in lymphocytes. The exercise\u2010induced increase of inflammatory genes methylation may be associated with immune function impairment during ageing.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Ageing\u2010induced increase in inflammatory gene expression through a reduction in DNA methylation might contribute to chronic diseases. Regular physical exercise practices, in turn, are associated with a decrease in the incidence of inflammatory diseases. We herein evaluated the effects of three exercise modalities on lymphocyte global and gene\u2010specific (interferon \u03b3 (IFN\u2010\u03b3) and interleukin 17A (IL\u201017A) DNA methylation in aged women (68\u00a0\u00b1\u00a07.5\u00a0years). This cross\u2010sectional study included 86 women, divided into four groups according to the physical exercise practice: 20 were practicing resistance training (RT); 24 were practicing water aerobics exercise (W); 22 were practicing water aerobics and resistance exercise (RWT), and 20 did not practice any physical exercise (CON). We evaluated volunteer functional capability using the Timed Up and Go (TUG) test, global lymphocyte DNA methylation by enzyme\u2010linked immunosorbent assay, IFN\u2010\u03b3 and IL\u201017A methylation by qPCR and CD4<jats:sup>+<\/jats:sup>IFN\u2010\u03b3<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup>IL\u201017<jats:sup>+<\/jats:sup> cell percentage by flow cytometry. The three physically exercised groups performed functional capability tests in a shorter period and showed a higher global lymphocyte DNA methylation and methylated CpGs of IL\u201017A and IFN\u2010\u03b3 promoter regions than the control group. The practice of resistance training (RT and RWT groups) lead to high global DNA methylation. The combination of resistance training and aerobic exercise led to the increase of lymphocyte IL\u201017A and IFN\u2010\u03b3 gene methylation induced by each separately. However, the percentage of IFN\u2010\u03b3<jats:sup>+<\/jats:sup> and IL\u201017<jats:sup>+<\/jats:sup> cells was lower only in the RT group. The exercise\u2010induced increase of inflammatory\u2010gene methylation may be associated with gene expression changes and immune function impairment during ageing.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep089673","type":"journal-article","created":{"date-parts":[[2021,7,6]],"date-time":"2021-07-06T22:38:46Z","timestamp":1625611126000},"page":"1878-1885","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Physical exercise increases global and gene\u2010specific (interleukin\u201017 and interferon\u2010\u03b3) DNA methylation in lymphocytes from aged women"],"prefix":"10.1113","volume":"106","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1808-5068","authenticated-orcid":false,"given":"Ot\u00e1vio A. S.","family":"Machado","sequence":"first","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"},{"name":"Physiology and Metabolism Study Center Physical Education Faculty of Sorocaba YMCA  Sorocaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4329-848X","authenticated-orcid":false,"given":"Vinicius L. S.","family":"Diniz","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E. P.","family":"Passos","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heloisa H.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laiane C.","family":"Santos\u2010Oliveira","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda L.","family":"Alecrim","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Bertola Lobato","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richelieau","family":"Manoel","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilana","family":"Correa","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane B.","family":"Silva","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"de Oliveira Poma","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Mendes de Almeida","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania C.","family":"Pithon\u2010Curi","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susana","family":"Diniz","sequence":"additional","affiliation":[{"name":"Anhanguera University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana C.","family":"Levada\u2010Pires","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Curi","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laureane Nunes","family":"Masi","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandro M.","family":"Hirabara","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9655-1477","authenticated-orcid":false,"given":"Renata","family":"Gorj\u00e3o","sequence":"additional","affiliation":[{"name":"Interdisciplinary Post\u2010Graduate Program in Health Sciences, Health Sciences department Cruzeiro do Sul University  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e3181915670"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1249\/MSS.0b013e3181a0c95c"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1004604"},{"key":"e_1_2_9_5_1","volume-title":"Borg's Perceived exertion and pain scales","author":"Borg G.","year":"1998"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glv156"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1089\/rej.2012.1397"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0503335102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.MSS.0000078924.61453.FB"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2013.01.008"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40279-013-0114-1"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00878.2014"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.8.e32"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500398102"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0802960"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2010.07508.x"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2008.08.006"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120658109"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0894"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00227-8"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1000533"},{"key":"e_1_2_9_22_1","volume-title":"Anthropometric standardization reference manual","author":"Lohman T. G.","year":"1988"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00134014"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/gly165"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2019.01.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/14779072.2019.1561278"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06105.x"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-1309-2037"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/44.8.1748"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01489.x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj461"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.2147\/ciia.2007.2.1.3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11914-010-0035-y"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2318-7-1"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0053938"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1503\/cmaj.051351"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075197-200011000-00011"},{"key":"e_1_2_9_38_1","unstructured":"World Health Organization(2000).Obesity: Preventing and managing the global epidemic. Report of a WHO consultation. World Health Organization Technical Report Series894. Geneva: World Health Organization."},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.7603\/s40681-015-0001-1"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP089673","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP089673","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP089673","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T12:41:45Z","timestamp":1693312905000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP089673"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,7,22]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2021,9]]}},"alternative-id":["10.1113\/EP089673"],"URL":"https:\/\/doi.org\/10.1113\/ep089673","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,7,22]]},"assertion":[{"value":"2021-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}