{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:38:15Z","timestamp":1783953495589,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T00:00:00Z","timestamp":1637625600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item>\n<jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>How does long non\u2010coding RNA myocardial infarction\u2010associated transcript (lncRNA MIAT) function in hypoxia\u2010induced H9C2 cells?<\/jats:p>\n<\/jats:list-item>\n<jats:list-item>\n<jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>LncRNA MIAT inhibited transcription of calcitonin gene\u2010related peptide by binding to splicing factor\u00a01, thereby promoting hypoxia\u2010induced H9C2 cell pyroptosis. This study provides a new theoretical basis for the treatment of acute myocardial infarction by using lncRNA MIAT as a molecular target to mediate cardiomyocyte pyrodeath.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Hypoxia induces severe cardiomyocyte pyroptosis, contributing to acute myocardial infarction. The aim of this study was to analyse the molecular mechanism of long non\u2010coding RNA myocardial infarction\u2010associated transcript (lncRNA MIAT) in hypoxia\u2010induced H9C2 cell pyroptosis. A hypoxic H9C2 cell model was established. Cell viability was detected via the Cell Counting Kit\u20108 method. Levels of lactate dehydrogenase, malondialdehyde and superoxide dismutase and expressions of pyroptotic markers, lncRNA MIAT, splicing factor\u00a01 (<jats:italic>SF1<\/jats:italic>) and calcitonin gene\u2010related peptide (<jats:italic>CGRP<\/jats:italic>) were detected via qRT\u2010PCR. The subcellular localization of lncRNA MIAT was predicted and confirmed via LncATLAS and nuclear\/cytosol fractionation assay. The binding relationships between lncRNA MIAT and <jats:italic>SF1<\/jats:italic> and between <jats:italic>SF1<\/jats:italic> and the <jats:italic>CGRP<\/jats:italic> promotor were verified via RNA immunoprecipitation. Rescue experiments were designed to confirm the role of lncRNA MIAT\/<jats:italic>SF1<\/jats:italic>\/<jats:italic>CGRP<\/jats:italic> in H9C2 cell pyroptosis. LncRNA MIAT was overexpressed in hypoxia\u2010induced H9C2 cells. Hypoxia induced pyroptosis in H9C2 cells. Silencing of lncRNA MIAT enhanced cell viability and alleviated pyroptosis. LncRNA MIAT inhibited <jats:italic>CGRP<\/jats:italic> transcription via binding to <jats:italic>SF1<\/jats:italic>. Overexpression of <jats:italic>SF1<\/jats:italic> promoted <jats:italic>CGRP<\/jats:italic> transcription and relieved H9C2 cell pyroptosis. Downregulation of <jats:italic>CGRP<\/jats:italic> reversed the role of silencing lncRNA MIAT in H9C2 cell pyroptosis. Overall, lncRNA MIAT inhibited <jats:italic>CGRP<\/jats:italic> transcription via binding to <jats:italic>SF1<\/jats:italic>, thereby promoting hypoxia\u2010induced H9C2 cell pyroptosis.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep089833","type":"journal-article","created":{"date-parts":[[2021,10,29]],"date-time":"2021-10-29T11:19:40Z","timestamp":1635506380000},"page":"58-67","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["LncRNA MIAT promotes hypoxia\u2010induced H9C2 cell pyroptosis via binding to <i>SF1<\/i> to inhibit <i>CGRP<\/i> transcription"],"prefix":"10.1113","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8000-9176","authenticated-orcid":false,"given":"Jingli","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Cardiology Shanxi Provincial People's Hospital  Taiyuan City Shanxi Province 030000 PR 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menthol attenuates inflammation and cardiac remodeling after myocardial infarction via the transient receptor potential melastatin\u00a08","volume":"33","author":"Wang Q.","year":"2020","journal-title":"American Journal of Hypertension"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1139\/bcb-2020-0672"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2020.108249"},{"key":"e_1_2_8_28_1","first-page":"533","article-title":"Knockdown of lncRNA MIAT inhibits proliferation and cisplatin resistance in non\u2010small cell lung cancer cells by increasing miR\u2010184 expression","volume":"19","author":"Wu L.","year":"2020","journal-title":"Oncology 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