{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:48:07Z","timestamp":1791582487948,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T00:00:00Z","timestamp":1651795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>Can an anaemic state modify adiposity and metabolic parameters in hypothalamic obese rats?<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>Hypothalamic obese rats do not display iron deficiency. However, the pharmacological induction of anaemia in hypothalamic obese rats resulted in reduced adiposity, characterized by a decrease in subcutaneous white and brown adipose tissue depots. These findings suggest that iron imbalance in obesity may elevate lipolysis.<\/jats:p><\/jats:list-item><\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Iron imbalance is frequent in obesity. Herein, we evaluated the impact of anaemia induced by phenylhydrazine on adiposity and metabolic state of hypothalamic obese rats. Hypothalamic obesity was induced by high doses of monosodium glutamate (MSG; 4\u00a0g\/kg) administered to neonatal male rats (<jats:italic>n<\/jats:italic>\u00a0=\u00a020). Controls (CTL; non\u2010obese rats) received equimolar saline (<jats:italic>n<\/jats:italic>\u00a0=\u00a020). Rats were weaned at 21\u00a0days of life. At 70\u00a0days, half of the rats received three intraperitoneal doses of phenylhydrazine (PHZ; 40\u00a0mg\/kg\/dose) or saline solution. Body weight and food intake were followed for 4\u00a0weeks after PHZ administration. At 92\u00a0days, rats were killed and blood was collected for microcapillary haematocrit (Hct) analysis and plasma quantification of glucose, triglycerides, total cholesterol and iron levels. The liver, the spleen, and the white (WAT) and brown (BAT) adipose tissues were excised, weighed and used for histology. MSG\u2010treated rats developed obesity, hypertriglyceridaemia and insulin resistance, compared to CTL rats, without changes in iron levels and Hct. PHZ administration reduced plasma iron levels and promoted similar tissue injuries in the spleen and liver from MSG and CTL rats. However, in MSG\u2010treated rats, PHZ decreased fasting glucose levels and Hct, as well as diminishing the subcutaneous WAT and BAT mass. Although MSG\u2010obesity does not affect plasma iron levels and Hct by itself, PHZ\u2010induced anaemia associated with obesity induces a marked drop in subcutaneous WAT and BAT mass, suggesting that iron imbalance may lead to increased lipolytic responses in obese rats, compared to lean rats.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep089883","type":"journal-article","created":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T11:07:08Z","timestamp":1649502428000},"page":"575-588","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Phenylhydrazine\u2010induced anaemia reduces subcutaneous white and brown adipose tissues in hypothalamic obese rats"],"prefix":"10.1113","volume":"107","author":[{"given":"Domwesley Wendreo","family":"de Souza","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Bioci\u00eancias e Sa\u00fade CCBS Universidade Estadual do Oeste do Paran\u00e1 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Enfermagem CCBS \u2010 Universidade Estadual do Oeste do Paran\u00e1 (Unioeste)  Cascavel Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliane Zanon","family":"Nenev\u00ea","sequence":"additional","affiliation":[{"name":"Gradua\u00e7\u00e3o Enfermagem CCBS \u2010 Universidade Estadual do Oeste do Paran\u00e1 (Unioeste)  Cascavel Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Paulo de Amorin","family":"Arruda","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Odontologia CCBS \u2010 Universidade Estadual do Oeste do Paran\u00e1 (Unioeste)  Cascavel Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Franciesco","family":"Vettorazzi","sequence":"additional","affiliation":[{"name":"Grupo de Pesquisa em Obesidade, CCBS Universidade Estadual do Oeste do Paran\u00e1 (Unioeste)  Cascavel Paran\u00e1 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