{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:35:36Z","timestamp":1790868936451,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,3]],"date-time":"2021-11-03T00:00:00Z","timestamp":1635897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870131"],"award-info":[{"award-number":["31870131"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p><jats:p>The physiological function of Mas\u2010related G protein\u2010coupled receptor D (MrgprD) in gastrointestinal motility is unknown. The aim of this study was to assess the effects of MrgprD and its receptor agonists on murine gastrointestinal motility.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p><jats:p><jats:italic>Mrgprd<\/jats:italic> deficiency improved murine gastrointestinal motility in vivo but had no effects on the spontaneous contractions of murine intestinal rings <jats:italic>ex vivo<\/jats:italic>. Systemic administration of the MrgprD ligand, either \u03b2\u2010alanine or alamandine, delayed gastrointestinal transit in vivo and attenuated the spontaneous contractions of isolated intestinal rings <jats:italic>ex vivo<\/jats:italic>.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Mas\u2010related G protein\u2010coupled receptor D (MrgprD) was first identified in sensory neurons of mouse dorsal root ganglion and has been demonstrated to be involved in sensations of pain and itch. Although expression of MrgprD has recently been found in the gastrointestinal (GI) tract, its physiological role in GI motility is unknown. To address this question, we used <jats:italic>Mrgprd<\/jats:italic> knockout (<jats:italic>Mrgprd<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup>) mice and MrgprD agonists to examine the effects of <jats:italic>Mrgprd<\/jats:italic> gene deletion and MrgprD signalling activation, respectively, on murine intestinal motility, both in vivo and <jats:italic>ex vivo<\/jats:italic>. We observed that the deletion of <jats:italic>Mrgprd<\/jats:italic> accelerated the transmission of charcoal through the mouse GI tract. But <jats:italic>Mrgprd<\/jats:italic> deficiency did not affect the mean amplitudes and frequencies of spontaneous contractions in ileum <jats:italic>ex vivo<\/jats:italic>. Colonic motor complexes in the proximal and the distal colon were recorded from wild\u2010type and <jats:italic>Mrgprd<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice, but their control frequencies were not different. Moreover, in wild\u2010type mice, systemic administration of an MrgprD agonist, either \u03b2\u2010alanine or alamandine, delayed GI transit in vivo and suppressed spontaneous contractions in the ileum and colonic motor complexes in the colon <jats:italic>ex vivo<\/jats:italic>. Our results suggest that MrgprD and its agonist are involved in the modulation of GI motility in mice.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep089958","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T06:10:02Z","timestamp":1634278202000},"page":"2502-2516","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Mas\u2010related G protein\u2010coupled receptor D is involved in modulation of murine gastrointestinal motility"],"prefix":"10.1113","volume":"106","author":[{"given":"Min","family":"Xu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life Sciences Nanjing Normal University  Nanjing Jiangsu PR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Molecular and Medical Biotechnology College of Life 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