{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:08:44Z","timestamp":1747188524039,"version":"3.40.5"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,11]],"date-time":"2022-05-11T00:00:00Z","timestamp":1652227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:sec><jats:title>New Findings<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:bold>What is the central question of this study?<\/jats:bold><\/jats:p>\n<jats:p>What are the early effects of dystrophin deficiency on sarcoplasmic reticulum Ca<jats:sup>2+<\/jats:sup> handling in the <jats:italic>mdx<\/jats:italic> mouse?<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p><jats:bold>What is the main finding and its importance?<\/jats:bold><\/jats:p>\n<jats:p>In the <jats:italic>mdx<\/jats:italic> mouse, Ca<jats:sup>2+<\/jats:sup> handling by the sarcoplasmic reticulum is little affected by the absence of dystrophin when looking at fibres without branches that have recently regenerated after massive myonecrosis. This has important implications for our understanding of Ca<jats:sup>2+<\/jats:sup> pathology in the <jats:italic>mdx<\/jats:italic> mouse.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>There is a variety of results in the literature regarding the effects of dystrophin deficiency on the Ca<jats:sup>2+<\/jats:sup> handling properties of the sarcoplasmic reticulum (SR) in the <jats:italic>mdx<\/jats:italic> mouse, an animal model of Duchenne muscular dystrophy. One possible source of variation is the presence of branched fibres. Fibre branching, a consequence of degenerative\u2013regenerative processes such as muscular dystrophy, has in itself a significant influence on the function of the SR. In this study, we attempted to detect early effects of dystrophin deficiency on SR Ca<jats:sup>2+<\/jats:sup> handling by using unbranched fibres from the immediate post\u2010necrotic stage in <jats:italic>mdx<\/jats:italic> mice (recently regenerated after massive necrosis). Using kinetically corrected fura\u20102 fluorescence signals measured during twitch and tetanus, we analysed the amplitude, rise time and decay time of \u0394[Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> in unfatigued and fatigued fibres. Decay was also resolved into SR pump and SR leak components. Fibres from <jats:italic>mdx<\/jats:italic> mice were similar in all respects to fibres from wild\u2010type littermates apart from: (1) a smaller amplitude of the initial spike of \u0394[Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> during a tetanus; and (2) a mitigation of the fall in \u0394[Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> amplitude during the course of fatigue. Our findings suggest that the early effects of a loss of dystrophin on SR Ca<jats:sup>2+<\/jats:sup> handling in <jats:italic>mdx<\/jats:italic> mice are subtle, and we emphasize the importance of distinguishing between Ca<jats:sup>2+<\/jats:sup> pathology that is attributable to lack of dystrophin and Ca<jats:sup>2+<\/jats:sup> pathology that is attributable to muscle degeneration.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep090057","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T13:26:23Z","timestamp":1650979583000},"page":"601-614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sarcoplasmic reticulum calcium handling in unbranched, immediately post\u2010necrotic fast\u2010twitch <i>mdx<\/i> fibres is similar to wild\u2010type littermates"],"prefix":"10.1113","volume":"107","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9511-4603","authenticated-orcid":false,"given":"Stephen","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Integrative Physiology School of Medicine Western Sydney University  Campbelltown New South Wales Australia"},{"name":"Department of Physiology, Faculty of Science Mahidol University  Ratchatewi Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sindy L. L.","family":"Kueh","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology School of Medicine Western Sydney University  Campbelltown New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W.","family":"Morley","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology School of Medicine Western Sydney University  Campbelltown New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stewart I.","family":"Head","sequence":"additional","affiliation":[{"name":"Department of Integrative Physiology School of Medicine Western Sydney University  Campbelltown New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1995.sp020883"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00007.2015"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199612)19:12<1549::AID-MUS4>3.0.CO;2-A"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(97)90029-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1916"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.65"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-009-9181-1"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.213314"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00128.2007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2010.056713"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1999.00417.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-002-0854-5"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13395-015-0041-y"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07815-7"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43844"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2008.03.008"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)83641-4"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019801"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2009.052019"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp017988"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1992.0058"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12366"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.160507"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00161.2017"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1991.sp018771"},{"key":"e_1_2_8_27_1","unstructured":"Kueh S. 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