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Inhibition of miRNA\u2010143\u20103p increased <jats:italic>Sox6<\/jats:italic> mRNA levels and reduced <jats:italic>Myh7<\/jats:italic> expression in cardiomyocytes, and prevented angiotensin II\u2010induced cardiomyocyte hypertrophy. The results indicate that the miRNA\u2010143\u20103p\u2013Sox6\u2013Myh7 pathway may play a key role in obesity\u2010induced cardiac hypertrophy.<\/jats:p>\n<\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec><jats:sec><jats:title>Abstract<\/jats:title><jats:p>Obesity induces cardiometabolic disorders associated with a high risk of mortality. We have previously shown that the microRNA (miRNA) expression profile is changed in obesity\u2010induced cardiac hypertrophy in male mice. Here, we investigated the effect of an obesogenic diet on the expression of miRNAs involved in cardiac hypertrophy in female mice. Female mice fed an obesogenic diet displayed an increased body weight gain, glucose intolerance, insulin resistance and dyslipidaemia. In addition, obese female mice exhibited cardiac hypertrophy associated with increased levels of several miRNAs, including miR\u2010143\u20103p. Bioinformatic analysis identified <jats:italic>Sox6<\/jats:italic>, regulator of <jats:italic>Myh7<\/jats:italic> gene transcription, as a predicted target of miR\u2010143\u20103p. Female mice fed an obesogenic diet exhibited decreased mRNA levels of <jats:italic>Sox6<\/jats:italic> and increased expression of <jats:italic>Myh7<\/jats:italic> in the heart. Loss\u2010of\u2010function studies in cardiomyocytes revealed that inhibition of miR\u2010143\u20103p increased <jats:italic>Sox6<\/jats:italic> mRNA levels and reduced <jats:italic>Myh7<\/jats:italic> expression. Collectively, our results indicate that obesity\u2010associated cardiac hypertrophy in female mice is accompanied by alterations in diverse miRNAs, and suggest that the miR\u2010143\u20103p\u2013Sox6\u2013Myh7 pathway may play a key role in obesity\u2010induced cardiac hypertrophy.<\/jats:p><\/jats:sec>","DOI":"10.1113\/ep090315","type":"journal-article","created":{"date-parts":[[2022,6,29]],"date-time":"2022-06-29T08:39:15Z","timestamp":1656491955000},"page":"892-905","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The miRNA\u2010143\u20103p\u2013Sox6\u2013Myh7 pathway is altered in obesogenic diet\u2010induced cardiac hypertrophy"],"prefix":"10.1113","volume":"107","author":[{"given":"T\u00e1batha","family":"de Oliveira Silva","sequence":"first","affiliation":[{"name":"Department of Anatomy Institute of Biomedical Sciences University of Sao Paulo  Sao Paulo 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