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These results support the use of\n                            <jats:italic>N<\/jats:italic>\n                            \u2010acetylcysteine to attenuate cardiovascular dysfunction induced by a \u2018Western\u2019 diet.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Individuals with overweight or obesity display respiratory and cardiovascular dysfunction, and oxidative stress is a causative factor in the general aetiology of obesity and of skeletal and cardiac muscle pathology. Thus, this preclinical study aimed to define diaphragmatic and cardiac morphological and functional alterations in response to an obesogenic diet in rats and the therapeutic potential of an antioxidant supplement,\n                      <jats:italic>N<\/jats:italic>\n                      \u2010acetylcysteine (NAC). Young male Wistar rats consumed ad libitum a \u2018lean\u2019 or high\u2010saturated fat, high\u2010sucrose (HFHS) diet for \u223c22\u00a0weeks and were randomized to control or NAC (2\u00a0mg\/ml in the drinking water) for the last 8 weeks of the dietary intervention. We then evaluated diaphragmatic and cardiac morphology and function. Neither HFHS diet nor NAC supplementation affected diaphragm\u2010specific force, peak power or morphology. Right ventricular weight normalized to estimated body surface area, left ventricular fractional shortening and posterior wall maximal shortening velocity were higher in HFHS compared with lean control animals and not restored by NAC. In HFHS rats, the elevated deceleration rate of early transmitral diastolic velocity was prevented by NAC. Our data showed that the HFHS diet did not compromise diaphragmatic muscle morphology or in vitro function, suggesting other possible contributors to breathing abnormalities in obesity (e.g., abnormalities of neuromuscular transmission). However, the HFHS diet resulted in cardiac functional and morphological changes suggestive of hypercontractility and diastolic dysfunction. Supplementation with NAC did not affect diaphragm morphology or function but attenuated some of the cardiac abnormalities in the rats receiving the HFHS diet.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1113\/ep090332","type":"journal-article","created":{"date-parts":[[2022,8,8]],"date-time":"2022-08-08T03:54:28Z","timestamp":1659930868000},"page":"1312-1325","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cardiac and respiratory muscle responses to dietary\n                    <i>N<\/i>\n                    \u2010acetylcysteine in rats consuming a high\u2010saturated fat, high\u2010sucrose diet"],"prefix":"10.1113","volume":"107","author":[{"given":"Rachel C.","family":"Kelley","sequence":"first","affiliation":[{"name":"Department of Applied Physiology and Kinesiology University of Florida  Gainesville Florida 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