{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T03:19:20Z","timestamp":1774149560928,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R01HL142483"],"award-info":[{"award-number":["R01HL142483"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["R21HL152112"],"award-info":[{"award-number":["R21HL152112"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microvascular perfusion of striated muscle is an important determinant of health throughout life. Birth is a transition with profound effects on the growth and function of striated muscle, but the regulation of microvascular perfusion around this transition is poorly understood. We used contrast\u2010enhanced ultrasound perfusion imaging (CEUS) to study the perfusion of left ventricular myocardium and hindlimb biceps femoris, which are populations of muscle with different degrees of change in pre\u2010 to postnatal workloads and different capacities for postnatal proliferative growth. We studied separate groups of lambs in late gestation (135\u00a0days\u2019 gestational age; 92% of term) and shortly after birth (5\u00a0days\u2019 postnatal age). We used CEUS to quantify baseline perfusion, perfusion during hyperaemia induced by adenosine infusion (myocardium) or electrically stimulated unloaded exercise (skeletal muscle), flow reserve and oxygen delivery. We found heart\u2010to\u2010body weight ratio was greater in neonates than fetuses. Microvascular volume and overall perfusion were lower in neonates than fetuses in both muscle groups at baseline and with hyperaemia. Flux rate differed with muscle group, with myocardial flux being faster in neonates than fetuses, but skeletal muscle flux being slower. Oxygen delivery to skeletal muscle at baseline was lower in neonates than fetuses, but was not significantly different in myocardium. Flow reserve was not different between ages. Given the significant somatic growth, and the transition from hyperplastic to hypertrophic myocyte growth occurring in the perinatal period, we postulate that the primary driver of lower neonatal striated muscle perfusion is faster growth of myofibres than their associated capillary networks.<\/jats:p>","DOI":"10.1113\/ep090809","type":"journal-article","created":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T13:31:17Z","timestamp":1669296677000},"page":"135-145","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Changes in microvascular perfusion of heart and skeletal muscle in sheep around the time of birth"],"prefix":"10.1113","volume":"108","author":[{"given":"Matthew W.","family":"Hagen","sequence":"first","affiliation":[{"name":"Center for Developmental Health Oregon Health &amp; Science University  Portland OR USA"},{"name":"Knight Cardiovascular Institute Oregon Health &amp; Science University  Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samantha","family":"Louey","sequence":"additional","affiliation":[{"name":"Center for Developmental Health Oregon Health &amp; Science University  Portland OR USA"},{"name":"Knight Cardiovascular Institute Oregon Health &amp; Science University  Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah M.","family":"Alaniz","sequence":"additional","affiliation":[{"name":"Center for Developmental Health Oregon Health &amp; Science University  Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todd","family":"Belcik","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute Oregon Health &amp; Science University  Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew M.","family":"Muller","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute Oregon Health &amp; Science University  Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Pediatrics University of Colorado Anschutz Medical Campus  Aurora CO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan R.","family":"Lindner","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute Oregon Health &amp; Science University  Portland OR USA"},{"name":"Division of Cardiovascular Medicine University of Virginia Medical Center  Charlottesville VA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1097-2562","authenticated-orcid":false,"given":"Sonnet S.","family":"Jonker","sequence":"additional","affiliation":[{"name":"Center for Developmental Health Oregon Health &amp; Science University  Portland OR USA"},{"name":"Knight Cardiovascular Institute Oregon Health &amp; Science University  Portland OR USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,24]]},"reference":[{"issue":"3","key":"e_1_2_9_2_1","first-page":"641","article-title":"A stereological method for estimating the total number of ventricular myocyte nuclei in fetal and postnatal hearts","volume":"187","author":"Austin A.","year":"1995","journal-title":"Journal of Anatomy"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-13-0567"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2016.03.017"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.10110"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.2000.00704.x"},{"issue":"1","key":"e_1_2_9_7_1","first-page":"397","article-title":"Developmental conditioning of the vasculature","volume":"5","author":"Clough G. 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