{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T07:15:07Z","timestamp":1760426107246,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T00:00:00Z","timestamp":1673222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000196","name":"Canada Foundation for Innovation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This retrospective exploratory analysis aimed to improve our understanding of the effect of passive heat stress and subsequent heat acclimation on the circulating concentration of extracellular vesicles (EVs). Healthy young adults (four females and six males, 25\u00a0\u00b1\u00a04 years of age, 1.72\u00a0\u00b1\u00a00.08\u00a0m in height and weighing 71.6\u00a0\u00b1\u00a09.0\u00a0kg) were heated with a water\u2010perfused suit before and after seven consecutive days of hot water immersion. Pre\u2010acclimation, participants were heated until oesophageal temperature increased to \u223c1.4\u00b0C above baseline values. Post\u2010acclimation, participants were heated until oesophageal temperature reached the same absolute value as the pre\u2010acclimation visit (\u223c38.2\u00b0C). Venous blood samples were obtained before and at the end of passive heating to quantify plasma concentrations of EVs from all cell types (CSFE<jats:sup>+<\/jats:sup>), all cell types except erythrocytes (CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>), platelets (CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>CD41<jats:sup>+<\/jats:sup>), endothelial cells (CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>CD62e<jats:sup>+<\/jats:sup>), red blood cells (CSFE<jats:sup>+<\/jats:sup>CD235a<jats:sup>+<\/jats:sup>) and leucocytes (CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>CD45<jats:sup>+<\/jats:sup>) via flow cytometry. Passive heat stress increased the concentration of CFSE<jats:sup>+<\/jats:sup>EVs (46,150,000\/ml [3,620,784, 88,679,216],<jats:italic>P<\/jats:italic>\u00a0=\u00a00.036), CFSE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>EVs (28,787,500\/ml [9,851,127, 47,723,873],<jats:italic>P<\/jats:italic>\u00a0=\u00a00.021) and CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>CD41<jats:sup>+<\/jats:sup>EVs (28,343,500\/ml [9,637,432, 47,049,568],<jats:italic>P<\/jats:italic>\u00a0=\u00a00.008). The concentration of CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>CD62e<jats:sup>+<\/jats:sup>EVs (94,230\/ml [\u221255,099, 243,559],<jats:italic>P<\/jats:italic>\u00a0=\u00a00.187), CSFE<jats:sup>+<\/jats:sup>CD235a<jats:sup>+<\/jats:sup>EVs (\u22121,414\/ml [\u221215,709, 12,882],<jats:italic>P<\/jats:italic>\u00a0=\u00a00.403) or CSFE<jats:sup>+<\/jats:sup>MHCI<jats:sup>+<\/jats:sup>CD45<jats:sup>+<\/jats:sup>EVs (\u2212192,915\/ml [\u2212690,166, 304,336],<jats:italic>P<\/jats:italic>\u00a0=\u00a00.828) did not differ during heat stress. The change in circulating EVs during passive heat stress did not differ after heat acclimation (thermal state \u00d7 acclimation interactions, all<jats:italic>P<\/jats:italic>\u00a0\u2265\u00a00.180). These results demonstrate that passive heat stress increases the circulating concentration of total and platelet EVs and that passive heat acclimation does not alter this increase.<\/jats:p>","DOI":"10.1113\/ep090823","type":"journal-article","created":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T09:46:20Z","timestamp":1673257580000},"page":"344-352","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impact of passive heat stress and passive heat acclimation on circulating extracellular vesicles: An exploratory analysis"],"prefix":"10.1113","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4894-9552","authenticated-orcid":false,"given":"Nicholas","family":"Ravanelli","sequence":"first","affiliation":[{"name":"School of Kinesiology Lakehead University Thunder Bay Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadiatou","family":"Barry","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute Montreal Quebec Canada"},{"name":"Department of Pharmacology and Physiology Universit\u00e9 de Montr\u00e9al Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7910-6921","authenticated-orcid":false,"given":"Anthony R.","family":"Bain","sequence":"additional","affiliation":[{"name":"Department of Kinesiology University of Windsor Windsor Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Vachon","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute Montreal Quebec Canada"},{"name":"Department of Medicine Universit\u00e9 de Montr\u00e9al Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Martel","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute Montreal Quebec Canada"},{"name":"Department of Medicine Universit\u00e9 de Montr\u00e9al Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5396-9489","authenticated-orcid":false,"given":"Daniel","family":"Gagnon","sequence":"additional","affiliation":[{"name":"Montreal Heart Institute Montreal Quebec Canada"},{"name":"Department of Pharmacology and Physiology Universit\u00e9 de Montr\u00e9al Montreal Quebec Canada"},{"name":"School of Kinesiology and Exercise Science Universit\u00e9 de Montr\u00e9al Montreal Quebec Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,1,9]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"499","article-title":"Insights into the role of heat shock protein 72 to whole\u2010body heat acclimation in humans","volume":"2","author":"Amorim F. 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