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However, it is unknown whether the rate of intestinal glucose absorption also changes. We therefore mounted small intestinal sections from C57BL\/6 female mice (8\u20139\u00a0weeks old) in Ussing chambers and measured active <jats:italic>ex vivo<\/jats:italic> glucose transport via the change in short\u2010circuit current (\u2206<jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub>) induced by glucose. Tissue viability was confirmed by a positive \u2206<jats:italic>I<\/jats:italic><jats:sub>sc<\/jats:sub> response to 100\u00a0\u00b5M carbachol following each experiment. Active glucose transport, assessed after addition of 5, 10, 25 or 45\u00a0mM <jats:sc>d<\/jats:sc>\u2010glucose to the mucosal chamber, was highest at 45\u00a0mM glucose in the distal jejunum compared to duodenum and ileum (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Incubation with the sodium\u2013glucose cotransporter 1 (SGLT1) inhibitor phlorizin reduced active glucose transport in a dose\u2010dependent manner in all regions (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). Active glucose uptake induced by addition of 45\u00a0mM glucose to the mucosal chamber in the absence or presence of phlorizin was assessed in jejunum at each oestrous cycle stage (<jats:italic>n<\/jats:italic>\u00a0=\u00a09\u201310 mice per stage). Overall, active glucose uptake was lower at oestrus compared to pro\u2010oestrus (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.025). This study establishes an <jats:italic>ex vivo<\/jats:italic> method to measure region\u2010specific glucose transport in the mouse small intestine. Our results provide the first direct evidence that SGLT1\u2010mediated glucose transport in the jejunum changes across the ovarian cycle. The mechanisms underlying these adaptations in nutrient absorption remain to be elucidated.<\/jats:p>","DOI":"10.1113\/ep091040","type":"journal-article","created":{"date-parts":[[2023,4,6]],"date-time":"2023-04-06T13:40:20Z","timestamp":1680788420000},"page":"865-873","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Active glucose transport varies by small intestinal region and oestrous cycle stage in mice"],"prefix":"10.1113","volume":"108","author":[{"given":"T. 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