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The purpose of this investigation was to determine if PGR impairs cardiac metabolism, specifically OxPhos. FVB (Friend Virus B\u2010type) mice were fed a normal\u2010protein (NP: 20% protein), or low\u2010protein (LP: 8% protein) isocaloric diet 2\u00a0weeks before mating. LP dams produce \u223c20% less milk, and pups nursed by LP dams experience reduced growth into adulthood as compared to pups nursed by NP dams. At birth (PN1), pups born to dams fed the NP diet were transferred to LP dams (PGR group) or a different NP dam (control group: CON). At weaning (PN21), all mice were fed the NP diet. At PN22 and PN80, mitochondria were isolated for respirometry (oxygen consumption rate, ) and fluorimetry (reactive oxygen species emission, ) analysis measured as baseline respiration (LEAK) and with saturating ADP (OxPhos). Western blotting at PN22 and PN80 determined protein abundance of uncoupling protein 3, peroxiredoxin\u20106, voltage\u2010dependent anion channel and adenine nucleotide translocator 1 to provide further insight into mitochondrial function. ANOVAs with the main effects of diet, sex and age with \u03b1\u2010level of 0.05 was set a priori. Overall, PGR (7.8\u00a0\u00b1\u00a01.1) had significant (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.01) reductions in respiratory control in complex I when compared to CON (8.9\u00a0\u00b1\u00a01.0). In general, our results show that PGR led to higher electron leakage in the form of free radical production and reactive oxygen species emission. No significant diet effects were found in protein abundance. The observed reduced respiratory control and increased ROS emission in PGR mice may increase risk for CVD in mice.<\/jats:p>","DOI":"10.1113\/ep091304","type":"journal-article","created":{"date-parts":[[2024,1,5]],"date-time":"2024-01-05T13:27:23Z","timestamp":1704461243000},"page":"562-575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Postnatal growth restriction alters myocardial mitochondrial energetics in mice"],"prefix":"10.1113","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8490-6111","authenticated-orcid":false,"given":"Joseph R.","family":"Visker","sequence":"first","affiliation":[{"name":"The Nora Eccles Harrison Cardiovascular Research and Training Institute University of Utah  Salt Lake City Utah USA"},{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric C.","family":"Leszczynski","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Austin G.","family":"Wellette\u2010Hunsucker","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"},{"name":"Department of Physiology University of Kentucky  Lexington Kentucky USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley C.","family":"McPeek","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa A.","family":"Quinn","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seong Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason N.","family":"Bazil","sequence":"additional","affiliation":[{"name":"Department of Physiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1203-0920","authenticated-orcid":false,"given":"David P.","family":"Ferguson","sequence":"additional","affiliation":[{"name":"Department of Kinesiology Michigan State University  East Lansing Michigan USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,1,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneumeth.2008.05.003"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.119.044452"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2009.05.011"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1997.tb18351.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(99)00258-7"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2007.01809.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.298.6673.564"},{"issue":"8663","key":"e_1_2_9_9_1","first-page":"8577","article-title":"Weight in infancy and death from ischaemic heart disease","volume":"2","author":"Barker D. 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