{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:58:22Z","timestamp":1778698702956,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T00:00:00Z","timestamp":1710288000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ischaemic heart diseases (IHD) are among the major causes of mortality in the elderly population. Although timely reperfusion is a common treatment for IHD, it causes additional damage to the ischaemic myocardium known as ischaemia\u2013reperfusion (IR) injury. Considering the importance of preventing reperfusion injuries, we aimed to examine the combination effect of mitochondrial transplantation (MT) and coenzyme Q<jats:sub>10<\/jats:sub> (CoQ<jats:sub>10<\/jats:sub>) in myocardial IR injury of aged male rats. Seventy\u2010two aged male Wistar rats were randomly divided into six groups: Sham, IR, CoQ<jats:sub>10<\/jats:sub>, MT, combination therapy (MT\u00a0+\u00a0CoQ<jats:sub>10<\/jats:sub>) and vehicle. Myocardial IR injury was established by occlusion of the left anterior descending coronary artery followed by reopening. Young male Wistar rats were used as mitochondria donors. Isolated mitochondria were injected intraventricularly (500\u00a0\u00b5L of a respiration buffer containing 6\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> \u00b1 5\u00a0\u00d7\u00a010<jats:sup>5<\/jats:sup>\u00a0mitochondria\/mL) in MT\u2010receiving groups at the onset of reperfusion. CoQ<jats:sub>10<\/jats:sub>\u00a0(10\u00a0mg\/kg\/day) was injected intraperitoneally for 2\u00a0weeks before IR induction. Twenty\u2010four hours after reperfusion, haemodynamic parameters, myocardial infarct size (IS), lactate dehydrogenase (LDH) release and cardiac mitochondrial function (mitochondrial reactive oxygen species (ROS) generation and membrane potential) were measured. The combination of MT and CoQ<jats:sub>10<\/jats:sub>\u00a0improved haemodynamic index changes and reduced IS and LDH release (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). It also decreased mitochondrial ROS generation and increased membrane potential (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.05). CoQ<jats:sub>10<\/jats:sub> also showed a significant cardioprotective effect. Combination therapy displayed greater cardioprotective effects than single treatments. This study revealed that MT and CoQ<jats:sub>10<\/jats:sub> combination treatment can be considered as a promising cardioprotective strategy to reduce myocardial IR injury in ageing, in part by restoring mitochondrial function.<\/jats:p>","DOI":"10.1113\/ep091358","type":"journal-article","created":{"date-parts":[[2024,3,13]],"date-time":"2024-03-13T20:20:01Z","timestamp":1710361201000},"page":"744-754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Mitochondrial transplantation combined with coenzyme Q<sub>10<\/sub> induces cardioprotection and mitochondrial improvement in aged male rats with reperfusion injury"],"prefix":"10.1113","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6644-5219","authenticated-orcid":false,"given":"Soleyman","family":"Bafadam","sequence":"first","affiliation":[{"name":"Molecular Medicine Research Center, Biomedicine Institute Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Physiology, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3355-9869","authenticated-orcid":false,"given":"Behnaz","family":"Mokhtari","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Drug Applied Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manoucheher Seyedi","family":"Vafaee","sequence":"additional","affiliation":[{"name":"Psychiatry Research Unit  Odense Denmark"},{"name":"Department of Nuclear Medicine Odense University Hospital  Odense Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zohreh Zavvari","family":"Oscuyi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Drug Applied Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samira","family":"Nemati","sequence":"additional","affiliation":[{"name":"Physiology Research Center Semnan University of Medical Sciences  Semnan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8092-7820","authenticated-orcid":false,"given":"Reza","family":"Badalzadeh","sequence":"additional","affiliation":[{"name":"Molecular Medicine Research Center, Biomedicine Institute Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Department of Physiology, Faculty of Medicine Tabriz University of Medical Sciences  Tabriz Iran"},{"name":"Drug Applied Research Center Tabriz University of Medical Sciences  Tabriz Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,3,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2021.111521"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2011.04.038"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtemb.2014.11.006"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20170106"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.15171\/apb.2018.037"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160889"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2023.04.024"},{"issue":"1","key":"e_1_2_8_9_1","first-page":"1","article-title":"Coenzyme Q10 protects against acute consequences of experimental myocardial infarction in rats","volume":"7","author":"Eleawa S. 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