{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T16:54:57Z","timestamp":1787504097162,"version":"build-2736575974"},"reference-count":32,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:00:00Z","timestamp":1697241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Human umbilical artery (HUA) preparations are of particular importance for in vitro studies on isolated blood vessels because their sampling is not risky for the patient, and they can provide the closest possible impression of changes related to the uteroplacental circulation during pre\u2010eclampsia. Using organ bath techniques, useful experimental protocols are provided for measuring some pathophysiological phenomena in the vascular responses of HUAs. Several vasoconstrictors (serotonin, prostaglandin F and phenylephrine) and vasodilators (acetylcholine and minoxidil) were seleted for determination of their vasoactivity in HUAs. The role of L\u2010type voltage\u2010operated calcium channels and different types of potassium channels (K\n                    <jats:sub>ATP<\/jats:sub>\n                    , BK\n                    <jats:sub>Ca<\/jats:sub>\n                    and K\n                    <jats:sub>V<\/jats:sub>\n                    ) were assessed, as was the impact of homocysteine. Serotonin was confirmed to be the most potent vasoconstrictor, while acetylcholine and phenylephrine caused variability in the relaxation and contraction response of HUA, respectively. The observed increase in serotonin\u2010induced contraction and a decrease in minoxidil\u2010induced relaxation in the presence of homocysteine suggested its procontractile effect on HUA preparations. Using selective blockers, it was determined that K\n                    <jats:sub>ATP<\/jats:sub>\n                    and K\n                    <jats:sub>V<\/jats:sub>\n                    channels participate in the minoxidil\u2010induced relaxation, while L\u2010type voltage\u2010dependent Ca\n                    <jats:sup>2+<\/jats:sup>\n                    \u00a0channels play an important role in the serotonin\u2010induced contraction. The presented protocols reveal some of the methodological challenges related to HUA preparations and indicate potential outcomes in interpreting the vascular effects of the investigated substances, both in physiological conditions and in the homocysteine\u2010induced pre\u2010eclampsia model.\n                  <\/jats:p>","DOI":"10.1113\/ep091374","type":"journal-article","created":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T12:54:21Z","timestamp":1697288061000},"page":"1569-1578","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Methodological challenges in using human umbilical artery as a model for in vitro studies"],"prefix":"10.1113","volume":"108","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-8433-7808","authenticated-orcid":false,"given":"Milica","family":"Gaji\u0107 Boji\u0107","sequence":"first","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0110or\u0111e","family":"\u0110ukanovi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Marinkovi\u0107","sequence":"additional","affiliation":[{"name":"Department of Paediatrics University Clinical Centre of the Republic of Srpska  Banja Luka The Republic of Srpska Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Jovi\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milo\u0161 P.","family":"Stojiljkovi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"},{"name":"Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragan M.","family":"Djuric","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"},{"name":"Faculty of Medicine, Institute of Medical Physiology \u2018Richard Burian\u2019 University of Belgrade  Belgrade Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranko","family":"\u0160krbi\u0107","sequence":"additional","affiliation":[{"name":"Centre for Biomedical Research, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"},{"name":"Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine University of Banja Luka  Banja Luka The Republic of Srpska Bosnia and Herzegovina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1972.222.2.345"},{"issue":"1","key":"e_1_2_11_3_1","first-page":"40","article-title":"In vitro vasoactive effects of dexmedetomidine on isolated human umbilical arteries","volume":"120","author":"Arun O.","year":"2019","journal-title":"Bratislavske lekarske 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