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This study investigated whether media supplemented with plasma samples taken at rest (\u226530\u00a0h since the most recent exercise session) from men who were endurance\u2010trained (END), strength\u2010trained (STR) or recreationally active controls (CON) impacted the results of four assays that mimic hallmarks of cancer (proliferation, migration, extracellular matrix invasion and anoikis resistance) in the BT\u2010549 breast cancer cell line. Compared to control conditions of either serum\u2010free media or fetal bovine serum as appropriate, BT\u2010549 cells cultured with plasma\u2010supplemented media regardless of group resulted in greater cell proliferation (\u223c20\u201350%) and cell migration (\u223c15\u201320%), and lower extracellular matrix invasion (\u223c10\u201320%) and anoikis resistance (\u223c15\u201320%). Supplementing media with plasma from END or STR did not impact any outcomes of these assays compared to plasma from CON. Media supplemented with human plasma can impact functional assays reflective of cancer hallmarks in BT\u2010549 cells, but effects of exercise on proliferation, migration, extracellular matrix invasion and anoikis resistance were not evident in resting blood samples of individuals with a prior history of exercise training.<\/jats:p>","DOI":"10.1113\/ep091383","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T00:31:09Z","timestamp":1700785869000},"page":"1124-1133","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Functional assays reflective of cancer hallmarks in BT\u2010549 cells are not impacted by media supplemented with exercise\u2010trained plasma"],"prefix":"10.1113","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3491-0865","authenticated-orcid":false,"given":"Ian A. 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