{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T05:07:40Z","timestamp":1784610460437,"version":"3.55.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T00:00:00Z","timestamp":1711670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>It has been proposed that diuretics can improve renal tissue oxygenation through inhibition of tubular sodium reabsorption and reduced metabolic demand. However, the impact of clinically used diuretic drugs on the renal cortical and medullary microcirculation is unclear. Therefore, we examined the effects of three commonly used diuretics, at clinically relevant doses, on renal cortical and medullary perfusion and oxygenation in non\u2010anaesthetised healthy sheep. Merino ewes received acetazolamide (250\u00a0mg; <jats:italic>n<\/jats:italic>\u00a0=\u00a09), furosemide (20\u00a0mg; <jats:italic>n<\/jats:italic>\u00a0=\u00a010) or amiloride (10\u00a0mg; <jats:italic>n<\/jats:italic>\u00a0=\u00a07) intravenously. Systemic and renal haemodynamics, renal cortical and medullary tissue perfusion and , and renal function were then monitored for up to 8\u00a0h post\u2010treatment. The peak diuretic response occurred 2\u00a0h (99.4\u00a0\u00b1\u00a014.8\u00a0mL\/h) after acetazolamide, at which stage cortical and medullary tissue perfusion and  were not significantly different from their baseline levels. The peak diuretic response to furosemide occurred at 1\u00a0h (196.5\u00a0\u00b1\u00a012.3\u00a0mL\/h) post\u2010treatment but there were no significant changes in cortical and medullary tissue oxygenation during this period. However, cortical tissue  fell from 40.1\u00a0\u00b1\u00a03.8\u00a0mmHg at baseline to 17.2\u00a0\u00b1\u00a04.4\u00a0mmHg at 3\u00a0h and to 20.5\u00a0\u00b1\u00a05.3\u00a0mmHg at 6\u00a0h after furosemide administration. Amiloride did not produce a diuretic response and was not associated with significant changes in cortical or medullary tissue oxygenation. In conclusion, clinically relevant doses of diuretic agents did not improve regional renal tissue oxygenation in healthy animals during the 8\u00a0h experimentation period. On the contrary, rebound renal cortical hypoxia may develop after dissipation of furosemide\u2010induced diuresis.<\/jats:p>","DOI":"10.1113\/ep091479","type":"journal-article","created":{"date-parts":[[2024,3,29]],"date-time":"2024-03-29T17:14:05Z","timestamp":1711732445000},"page":"766-778","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effects of furosemide, acetazolamide and amiloride on renal cortical and medullary tissue oxygenation in non\u2010anaesthetised healthy sheep"],"prefix":"10.1113","volume":"109","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5402-1809","authenticated-orcid":false,"given":"Connie P. 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