{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T23:07:01Z","timestamp":1788822421324,"version":"build-2803163510"},"reference-count":87,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000341","name":"Arthritis Research UK","doi-asserted-by":"publisher","award":["19891"],"award-info":[{"award-number":["19891"]}],"id":[{"id":"10.13039\/501100000341","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This review highlights recent advancements in our understanding of muscle protein synthesis (MPS) across the lifespan, with a focus on dietary protein strategies to support muscle health. Given that skeletal muscle is crucial for whole\u2010body metabolism, movement and independence, maintaining muscle mass throughout life is essential. However, the gradual decline in muscle mass and strength with age, known as sarcopenia, represents a significant health concern. Muscle mass is regulated by the balance of MPS and muscle protein breakdown, with dietary protein intake playing a central role in stimulating MPS and maintaining a positive protein balance. Much of our current understanding of protein intake, specifically its quantity, quality and distribution, comes from stable isotope\u2010labelled amino acid methods. These techniques, however, are limited by time constraints and controlled settings, providing only brief snapshots of MPS dynamics. The use of deuterium oxide (D\u2082O) has provided new insights, enabling long\u2010term measures of muscle protein metabolism in free\u2010living conditions. Measurements of longer\u2010term MPS using D\u2082O suggest that older adults might benefit from protein intakes of &gt;1.2\u00a0g\/kg\/day to enhance MPS. Additionally, replacing protein in the diet with higher\u2010quality sources or enriching lower protein intakes with leucine can further increase MPS. Nevertheless, discrepancies remain regarding optimal protein requirements and the long\u2010term efficacy of supplementing with enriched suboptimal protein doses. The continued application of D\u2082O in dietary protein research has the potential to provide further insights into the prolonged effects of various protein strategies on muscle preservation across the lifespan.<\/jats:p>","DOI":"10.1113\/ep092016","type":"journal-article","created":{"date-parts":[[2025,4,25]],"date-time":"2025-04-25T05:55:18Z","timestamp":1745560518000},"page":"949-960","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Investigating muscle protein synthesis using deuterium oxide: The impact of dietary protein interventions across the lifespan"],"prefix":"10.1113","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9245-3095","authenticated-orcid":false,"given":"Matthew S.","family":"Brook","sequence":"first","affiliation":[{"name":"Medical Research Council Versus Arthritis Centre for Musculoskeletal Ageing Research University of Nottingham  Nottingham UK"},{"name":"NIHR Nottingham BRC University of Nottingham  Nottingham UK"},{"name":"School of Life Sciences University of Nottingham  Nottingham UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2010.29819"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2011.225003"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-009-0377-x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2015.05.021"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00488.2006"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1997.273.1.E122"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.0575f.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13005"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1113\/EP087492"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000392"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00157.2017"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP272857"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-273755"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12532"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-022-00651-y"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2005.12.023"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.420"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)61460-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajcnut.2024.08.030"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000001061"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afy169"},{"key":"e_1_2_9_23_1","first-page":"422","article-title":"Anabolic signaling deficits underlie amino acid resistance of wasting, aging muscle","volume":"18","author":"Cuthbertson D.","year":"2004","journal-title":"Federation of American Societies for Experimental Biology Journal"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP272472"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111938"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxy091"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqx028"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.12160"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00075197-200309000-00004"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00580.2004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo6040034"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-018-2640-5"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.90411.2008"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00579.2012"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mie.2015.05.018"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2024.09.012"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00610.2017"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnesp.2024.10.152"},{"key":"e_1_2_9_39_1","volume-title":"Introduction to body composition assessment using the deuterium dilution technique with analysis of saliva samples by Fourier transform infrared spectrometry","author":"(IAEA), I. A. E. A","year":"2010"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2000.89.1.81"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00360.2019"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqaa136"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2015.97"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.164483"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118803"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6622"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2016-0645"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/sms.14506"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajcnut.2024.05.009"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP275430"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00609.2014"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2012.00260"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2023.02.023"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glu103"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.26401"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxz211"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.116.136424"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1139\/apnm-2021-0806"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00425.2021"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/nqz332"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjnut.2023.11.004"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108295"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00271.2003"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)74342-1"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13539-013-0117-7"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcsm.13431"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2020.00025"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00125.2010"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1992.262.3.E372"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxab358"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1249\/01.mss.0000321893.46012.15"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jada.2009.06.369"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00076.2009"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00608.2019"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2008.26668"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.286.10.1206"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/78.2.250"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.85.12.4481"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140903"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.2009.27543"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21507"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutos.2021.02.005"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2017.09.008"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00650.2013"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2013.253203"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.055517"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/84.3.475"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2021.685165"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP092016","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T05:24:59Z","timestamp":1751347499000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP092016"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,24]]},"references-count":87,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1113\/EP092016"],"URL":"https:\/\/doi.org\/10.1113\/ep092016","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,24]]},"assertion":[{"value":"2024-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}