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Although the concentrations of some of these intermediates have been reported to be increased in large cohorts of septic patients, a detailed analysis of their changes during sepsis is still lacking. Here, we investigated the plasma concentrations of several TCA intermediates in a swine model of endotoxic shock and the relationship between these TCA cycle intermediates and lactate production. Nine female swine were administered lipopolysaccharide to induce endotoxic shock, while four females served as controls. Plasma samples were collected at three time points: baseline, 3 and 6\u00a0h after lipopolysaccharide administration. Control samples were collected at parallel time points. Quantification of TCA intermediates, lactate and pyruvate was performed by high\u2010performance liquid chromatography. Oxygen\u2010derived variables were obtained by gas analysis of arterial and venous samples. The endotoxic shock group showed a significant increase in lactate, accompanied by stability of oxygen\u2010derived variables and a low lactate:pyruvate ratio, indicative of aerobic conditions. Of all the TCA intermediates analysed, only citrate and succinate showed significant increases compared with controls. Furthermore, the changes in lactate were determined, in part, by the changes in succinate concentration. The increase in succinate concentrations was associated with the increase in lactate in global aerobic conditions. Our results suggest a potential role for succinate as a biomarker of aerobic lactate production.<\/jats:p>","DOI":"10.1113\/ep092109","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T18:35:34Z","timestamp":1742409334000},"page":"550-560","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Increase in plasma succinate is associated with aerobic lactate production in a model of endotoxic shock"],"prefix":"10.1113","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6488-806X","authenticated-orcid":false,"given":"Juan D.","family":"Caicedo Ruiz","sequence":"first","affiliation":[{"name":"Department of Intensive Care Fundaci\u00f3n Santa Fe de Bogot\u00e1  Bogot\u00e1 Colombia"},{"name":"Physiology Division Department of Physiological Sciences Faculty of Medicine Universidad Nacional de Colombia  Bogot\u00e1 Colombia"},{"name":"Department of Intensive Care Medicine Fundaci\u00f3n Valle del Lili  Cali Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge I.","family":"Alvarado Sanchez","sequence":"additional","affiliation":[{"name":"Department of Intensive Care Fundaci\u00f3n Santa Fe de Bogot\u00e1  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan J.","family":"Diaztagle Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Physiology Division Department of Physiological Sciences Faculty of Medicine Universidad Nacional de Colombia  Bogot\u00e1 Colombia"},{"name":"Fundaci\u00f3n Universitaria de Ciencias de la Salud, Department of Internal Medicine Hospital de San Jos\u00e9  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e1ndida","family":"Diaz Brochero","sequence":"additional","affiliation":[{"name":"Pontificia Universidad Javeriana, Department of Internal Medicine, Hospital Universitario San Ignacio  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis E.","family":"Cruz Martinez","sequence":"additional","affiliation":[{"name":"Physiology Division Department of Physiological Sciences Faculty of Medicine Universidad Nacional de Colombia  Bogot\u00e1 Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"issue":"4","key":"e_1_2_10_2_1","first-page":"621","article-title":"Unveiling the significance of dynamic arterial elastance: an insightful approach to assessing arterial load in an endotoxin shock model","volume":"60","author":"Alvarado S\u00e1nchez J. 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