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We hypothesise that sympathetic dysregulation observed in PD may involve pathological changes caused by \u03b1\u2010synuclein in stellate ganglia (SG). This study aimed to investigate \u03b1\u2010synucleinopathy in SG of the genetic PD murine animal model. Mice overexpressing Ala30Pro (A30P) mutant \u03b1\u2010synuclein were used. We here demonstrate a technique for meticulously dissecting SG. The collected SG from the transgenic mice were immunolabelled with neuronal markers, A30P human mutant \u03b1\u2010synuclein and anti\u2010\u03b1\u2010synuclein aggregates. A30P mutant \u03b1\u2010synuclein protein was expressed in the sympathetic neuronal (tyrosine hydroxylase (TH)\u2010positive) cell bodies. Approximately 27% of the TH\u2010positive cell bodies expressed the A30P mutant \u03b1\u2010synuclein protein. The mutant protein was densely localised at the cardiopulmonary pole of the SG. Additionally, we observed that the A30P mutant protein formed fibril aggregation in the SG. Our findings suggest that \u03b1\u2010synucleinopathy in the PD animal model can affect the sympathetic autonomic nervous system, providing insight for further research into targeting \u03b1\u2010synuclein pathology in the SG as a potential link between cardiac dysautonomia and PD.<\/jats:p>","DOI":"10.1113\/ep092261","type":"journal-article","created":{"date-parts":[[2025,2,22]],"date-time":"2025-02-22T05:34:41Z","timestamp":1740202481000},"page":"866-876","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intact microdissection of stellate ganglia in a Parkinson's disease model reveals aggregation of mutant human \u03b1\u2010synuclein in their cell bodies"],"prefix":"10.1113","volume":"110","author":[{"given":"Bonn","family":"Lee","sequence":"first","affiliation":[{"name":"School of Veterinary Medicine, Faculty of Health and Medical Sciences University of Surrey Guildford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiraz","family":"Ahmad","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculty of Health and Medical Sciences University of Surrey Guildford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte E.","family":"Edling","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, Faculty of Health and Medical Sciences University of Surrey Guildford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fiona E. 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