{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T02:40:29Z","timestamp":1790995229821,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T00:00:00Z","timestamp":1738108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Abundant evidence indicates that skeletal muscle plays a key role in regulating metabolic homeostasis. Therefore, maintaining healthy skeletal muscles is essential to good health. While prolonged muscle inactivity is known to cause oxidative stress and muscle loss, it remains unclear whether a shift from an active to a sedentary lifestyle induces similar effects. This study tested the hypothesis that transitioning to a sedentary lifestyle rapidly leads to oxidative stress and muscle loss in the load\u2010bearing soleus muscle. Adult Wistar rats were randomly divided into control (CON;\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a08) and sedentary (SED;\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a08) groups. During a 7\u2010day experimental period, CON rats were housed in standard cages allowing free movement, while SED rats were confined to smaller cages promoting sedentary behaviour. Soleus muscles were analysed for antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX)), as well as two oxidative stress biomarkers (advanced protein oxidation products (AOPPs) and 4\u2010hydroxynonenal (4\u2010HNE)). Sedentary behaviour caused a 17.2% reduction in the soleus\u2010to\u2010body weight ratio (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.001). Moreover, the activities of SOD, CAT and GPX were significantly lower in the soleus muscle of SED animals (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.05), while AOPPs and 4\u2010HNE levels were higher (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.001 and\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.05) compared to CON animals. These findings provide the first evidence that transitioning from an active to a sedentary lifestyle leads to the rapid onset of oxidative stress and atrophy in the soleus muscle. Importantly, the results suggest that impaired antioxidant defences contribute to sedentary behaviour\u2010induced oxidative stress in load\u2010bearing muscles.\n                  <\/jats:p>","DOI":"10.1113\/ep092331","type":"journal-article","created":{"date-parts":[[2025,1,30]],"date-time":"2025-01-30T02:00:14Z","timestamp":1738202414000},"page":"857-865","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Sedentary lifestyle induces oxidative stress and atrophy in rat skeletal muscle"],"prefix":"10.1113","volume":"110","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5037-0988","authenticated-orcid":false,"given":"Irem","family":"Gungor\u2010Orhan","sequence":"first","affiliation":[{"name":"Department of Exercise and Sport Sciences, Exercise and Sport Physiology Division, Faculty of Sport Sciences Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5729-5944","authenticated-orcid":false,"given":"Senay","family":"Akin","sequence":"additional","affiliation":[{"name":"Department of Exercise and Sport Sciences, Exercise and Sport Physiology Division, Faculty of Sport Sciences Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4545-7605","authenticated-orcid":false,"given":"Scott K.","family":"Powers","sequence":"additional","affiliation":[{"name":"Department of Applied Physiology and Kinesiology University of Florida  Gainesville Florida USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7288-7219","authenticated-orcid":false,"given":"Seda","family":"Olgaz\u2010Bingol","sequence":"additional","affiliation":[{"name":"Turkish Doping Control Center Hacettepe University  Ankara T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3334-4793","authenticated-orcid":false,"given":"Haydar A.","family":"Demirel","sequence":"additional","affiliation":[{"name":"Faculty of Sport Sciences Near East University  Nicosia Cyprus"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12902-022-01221-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00257.2016"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2013.06.011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.2040-4603.2012.tb00425.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eclinm.2022.101424"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.34067\/KID.0000000000000313"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4467"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2013.857291"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjsports-2017-098963"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00104.2006"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.30.18515"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000357332"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-022-12728-6"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCO.0000000000000283"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.01.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00126.2005"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2017.00184"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP284301"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00591.2011"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00767.2006"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12576-016-0444-5"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.5.2.7391"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11244005"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/JES.0b013e3181877d1a"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00734.2009"},{"key":"e_1_2_8_27_1","first-page":"S275","article-title":"Skeletal muscle as an endocrine organ: The role of myokines in muscle\u2010fat cross\u2010talk","volume":"59","author":"Pedersen B. 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