{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T12:44:29Z","timestamp":1763124269315,"version":"3.45.0"},"reference-count":92,"publisher":"Wiley","license":[{"start":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T00:00:00Z","timestamp":1763078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003981","name":"Agenzia Spaziale Italiana","doi-asserted-by":"publisher","award":["DC\u2010VUM\u20102017\u2010006"],"award-info":[{"award-number":["DC\u2010VUM\u20102017\u2010006"]}],"id":[{"id":"10.13039\/501100003981","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The effects of muscle disuse on the propagation of action potentials along motor unit (MU) muscle fibres, a key process for effective muscle activation and force generation, remain poorly understood. The aim of this study was to investigate changes in action potential propagation and to identify biological factors influencing these changes following unilateral lower\u2010limb suspension (ULLS) and active recovery (AR). Eleven young males underwent 10 days of ULLS followed by 21 days of AR involving resistance exercise. Maximal force of the knee extensors (MVC), high\u2010density surface EMG recordings and muscle biopsies of the vastus lateralis muscle were collected before ULLS, after ULLS and after AR. EMG recordings collected during submaximal isometric contractions were decomposed to estimate single\u2010MU conduction velocity (CV). Biopsies were used to measure muscle fibre diameters via histochemical analysis and ion channel transcriptomic profiles via mRNA sequencing. The MVC declined by 29% after ULLS and returned to baseline after AR. MU CV decreased after ULLS and recovered fully, even exceeding baseline values after AR. Muscle fibre diameters did not change across the interventions and showed no correlation with MU CV. Conversely, a feature importance analysis revealed that mRNA expression levels of specific ion channel genes, particularly those involved in K\n                    <jats:sup>+<\/jats:sup>\n                    transport, were correlated with MU CV at baseline and across the interventions. This study highlights the crucial role of K\n                    <jats:sup>+<\/jats:sup>\n                    ion channels in influencing MU CV in humans, offering new insights into MU CV modulation and the mechanisms of changes in muscle force after disuse and active recovery.\n                  <\/jats:p>","DOI":"10.1113\/ep093065","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T12:41:42Z","timestamp":1763124102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Changes in motor unit conduction velocity after unilateral lower\u2010limb suspension and active recovery are correlated with muscle ion channel gene expression"],"prefix":"10.1113","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5221-580X","authenticated-orcid":false,"given":"Giacomo","family":"Valli","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Sciences University of Brescia  Brescia Italy"},{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8572-5147","authenticated-orcid":false,"given":"Fabio","family":"Sarto","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9094-8932","authenticated-orcid":false,"given":"Francesco","family":"Negro","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Sciences University of Brescia  Brescia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3767-0855","authenticated-orcid":false,"given":"Elena","family":"Monti","sequence":"additional","affiliation":[{"name":"Baxter Laboratory for Stem Cell Biology, Department of Microbiology and Immunology Stanford School of Medicine  Stanford California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7005-8708","authenticated-orcid":false,"given":"Giuseppe","family":"Sirago","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"},{"name":"Institute of Sport Sciences and Department of Biomedical Sciences University of Lausanne  Lausanne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7556-6928","authenticated-orcid":false,"given":"Matteo","family":"Paganini","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6970-0011","authenticated-orcid":false,"given":"Sandra","family":"Zampieri","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"},{"name":"Department of Surgery, Oncology and Gastroenterology University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3165-4536","authenticated-orcid":false,"given":"Martino V.","family":"Franchi","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1901-3894","authenticated-orcid":false,"given":"Andrea","family":"Casolo","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5793-8989","authenticated-orcid":false,"given":"Juli\u00e1n","family":"Candia","sequence":"additional","affiliation":[{"name":"Longitudinal Studies Section, Translational Gerontology Branch, National Institute of Aging National Institutes of Health  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6273-1613","authenticated-orcid":false,"given":"Luigi","family":"Ferrucci","sequence":"additional","affiliation":[{"name":"Longitudinal Studies Section, Translational Gerontology Branch, National Institute of Aging National Institutes of Health  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0167-1845","authenticated-orcid":false,"given":"Marco V.","family":"Narici","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6855-9180","authenticated-orcid":false,"given":"Giuseppe","family":"De Vito","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padova  Padova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1113\/jphysiol.1987.sp016756","article-title":"Muscle fibre conduction velocity in motor units of the human anterior tibial muscle: A new size principle parameter","volume":"391","author":"Andreassen S.","year":"1987","journal-title":"The Journal of Physiology"},{"key":"e_1_2_10_3_1","unstructured":"Arendt\u2010Nielsen L. Forster A. &Mills K. R.(1984).The relationship between muscle\u2010fibre conduction velocity and force in the human vastus lateralis.https:\/\/api.semanticscholar.org\/CorpusID:113663259"},{"issue":"2","key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1097\/00004691-198904000-00004","article-title":"Measurement of muscle fiber conduction velocity in humans: Techniques and applications","volume":"6","author":"Arendt\u2010Nielsen L.","year":"1989","journal-title":"Journal of Clinical Neurophysiology"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"456","DOI":"10.3389\/fpsyg.2017.00456","article-title":"Repeated measures correlation","volume":"8","author":"Bakdash J. 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