{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T17:41:38Z","timestamp":1777311698359,"version":"3.51.4"},"reference-count":70,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T00:00:00Z","timestamp":1763769600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Endothelial dysfunction drives obesity\u2010related complications. Doppler ultrasound measurement of blood flow during 1\u2010min passive leg movements (PLM) is a valuable non\u2010invasive tool for assessing endothelial function and nitric oxide (NO)\u2010mediated vasodilation. The objectives of this work were t o identify endothelial dysfunction biomarkers in young individuals with obesity (OB) using the PLM test; to evaluate the effects of a rehabilitation programme on these biomarkers; and to explore associations between PLM data, oxidative metabolism and blood biomarkers of microvascular impairment. Fifteen male OB (age 17\u00a0\u00b1\u00a04\u00a0years; body mass 121.4\u00a0\u00b1\u00a024.1\u00a0kg; body mass index 39.3\u00a0\u00b1\u00a07.5\u00a0kg\u00a0m\n                    <jats:sup>\u22122<\/jats:sup>\n                    ) were tested before (PRE) and after (POST) a 3\u2010week multidisciplinary body mass reduction programme. Fifteen age\u2010matched normal\u2010weight males (CTRL) underwent PRE measurements. Participants performed an incremental exercise, a PLM test and underwent blood biomarker analysis. Peak oxygen uptake and ventilatory thresholds (mL kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u00a0min\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) were \u223c40% lower in OB versus CTRL (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.001) and improved by \u223c8% in OB POST versus PRE (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.05). Plasma nitrite concentration was lower in OB (0.18\u00a0\u00b1\u00a00.09 \u00b5mol L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) versus CTRL (0.51\u00a0\u00b1\u00a00.49;\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    =\u00a00.02). Baseline blood flow, normalized for appendicular muscle mass, was similar between the two groups, whereas peak blood flow, \u0394peak (difference between peak and baseline) and the area under the blood flow versus time curve were significantly lower in OB PRE, with improvements in OB POST. Several blood biomarkers of endothelial barrier function and permeability differed in OB versus CTRL. The blunted PLM\u2010induced hyperaemic response and lower plasma nitrite levels indicate impaired endothelial function in young individuals with obesity, occurring before the onset of cardiovascular and metabolic complications.\n                  <\/jats:p>","DOI":"10.1113\/ep093109","type":"journal-article","created":{"date-parts":[[2025,11,22]],"date-time":"2025-11-22T15:34:36Z","timestamp":1763825676000},"page":"1134-1149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Impaired nitric oxide\u2010dependent endothelial function in young male individuals with obesity before the onset of symptoms and complications"],"prefix":"10.1113","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2447-4684","authenticated-orcid":false,"given":"Lucrezia","family":"Zuccarelli","sequence":"first","affiliation":[{"name":"Department of Medicine University of Udine  Udine Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-9216","authenticated-orcid":false,"given":"Giovanni","family":"Baldassarre","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine  Udine Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adele","family":"Bondesan","sequence":"additional","affiliation":[{"name":"Experimental Laboratory for Auxo\u2010endocrinological Research Istituto Auxologico Italiano, IRCCS  Piancavallo\u2010Verbania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Caroli","sequence":"additional","affiliation":[{"name":"Experimental Laboratory for Auxo\u2010endocrinological Research Istituto Auxologico Italiano, IRCCS  Piancavallo\u2010Verbania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"De Micheli","sequence":"additional","affiliation":[{"name":"Experimental Laboratory for Auxo\u2010endocrinological Research Istituto Auxologico Italiano, IRCCS  Piancavallo\u2010Verbania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"Tringali","sequence":"additional","affiliation":[{"name":"Experimental Laboratory for Auxo\u2010endocrinological Research Istituto Auxologico Italiano, IRCCS  Piancavallo\u2010Verbania Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Favaretto","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Udine  Udine Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Suraj\u2010Prazmowska","sequence":"additional","affiliation":[{"name":"Jagiellonian Center for Experimental Therapeutics (JCET) Jagiellonian University  Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Kurpinska","sequence":"additional","affiliation":[{"name":"Jagiellonian Center for Experimental Therapeutics (JCET) Jagiellonian University  Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanna","family":"Majerczak","sequence":"additional","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences Jagiellonian University Medical College  Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Chlopicki","sequence":"additional","affiliation":[{"name":"Jagiellonian Center for Experimental Therapeutics (JCET) Jagiellonian University  Krakow Poland"},{"name":"Department of Pharmacology Jagiellonian University Medical College  Krakow Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1143-5590","authenticated-orcid":false,"given":"Jerzy A.","family":"Zoladz","sequence":"additional","affiliation":[{"name":"Chair of Exercise Physiology and Muscle Bioenergetics, Faculty of Health Sciences Jagiellonian 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