{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T01:48:53Z","timestamp":1784080133101,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AG064016"],"award-info":[{"award-number":["R01AG064016"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["TL1TR002371"],"award-info":[{"award-number":["TL1TR002371"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Elastin insufficiency is associated with structural differences in the large elastic arteries and cerebral artery dysfunction. However, previous studies have not assessed potential sex differences in cerebrovascular function. We measured cerebral blood flow (CBF) using arterial spin labeling MRI at rest and in response to hypercapnia challenge (cerebrovascular responsiveness, CR) in middle\u2010aged and old elastin haploinsufficient (\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    ) and wild\u2010type (\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    ) mice. We also assessed neuroinflammation by microglia and astrocyte cell counts. We found that\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice had a significantly lower resting CBF in the cerebral cortex compared with\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice, with similar non\u2010significant trends in the hippocampus and thalamus. In contrast, the\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice had an intact hypercapnic response, resulting in better CR compared with\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    in hippocampus, with a similar trend in the cerebral cortex. Sex did not impact CBF or CR. We found that\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice had lower hippocampal volume compared with\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice. Glia cell counts were highly dependent on brain region, with\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                    <\/jats:italic>\n                    mice having more microglia in the cerebral cortex, but fewer astrocytes in the hippocampus compared with\n                    <jats:italic>\n                      Eln\n                      <jats:sup>+\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice. While sex also impacted glial cell counts, we found no interactions between sex and\n                    <jats:italic>Eln<\/jats:italic>\n                    genotype. Our results demonstrate that elastin haploinsufficiency results in lower resting CBF, but greater CR.\n                  <\/jats:p>","DOI":"10.1113\/ep093234","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:10:09Z","timestamp":1769505009000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lower cerebral blood flow but not cerebrovascular response in elastin haploinsufficient mice"],"prefix":"10.1113","author":[{"given":"Abigail E.","family":"Cullen","sequence":"first","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily H.","family":"Reeve","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nick R.","family":"Winder","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant D.","family":"Henson","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayantara","family":"Arora","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Leonhardt","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ainsley","family":"Hogan","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahana Krishna","family":"Kumaran","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naly","family":"Setthavonsack","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, School of Medicine Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Krajbich","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, School of Medicine Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Art","family":"Riddle","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Zimmerman","sequence":"additional","affiliation":[{"name":"Helfgott Research Institute National University of Natural Medicine  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nabil J.","family":"Alkayed","sequence":"additional","affiliation":[{"name":"Knight Cardiovascular Institute Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin M.","family":"Pike","sequence":"additional","affiliation":[{"name":"Biomedical Engineering, Advanced Imaging Research Center Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randall L.","family":"Woltjer","sequence":"additional","affiliation":[{"name":"Pathology and Laboratory Medicine, School of Medicine Oregon Health and Science University  Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley E.","family":"Walker","sequence":"additional","affiliation":[{"name":"Human Physiology University of Oregon  Eugene Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16668536"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2024.1347987"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2010.09.025"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nicl.2013.08.017"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","DOI":"10.3389\/fncel.2022.893030","article-title":"GPR39 deficiency impairs memory and alters oxylipins and inflammatory cytokines without affecting cerebral blood flow in a high\u2010fat diet mouse model of cognitive impairment","volume":"16","author":"Bah T. M.","year":"2022","journal-title":"Frontiers in Cellular Neuroscience"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2012.03.021"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awac257"},{"issue":"1","key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1186\/s12974-022-02604-w","article-title":"Alzheimer's disease\u2010related transcriptional sex differences in myeloid cells","volume":"19","author":"Coales I.","year":"2022","journal-title":"Journal of Neuroinflammation"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.70688"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01588-0"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201709069"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00517.2020"},{"issue":"18","key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"4860","DOI":"10.1523\/JNEUROSCI.1020-17.2017","article-title":"A critical role for astrocytes in hypercapnic vasodilation in brain","volume":"37","author":"Howarth E.","year":"2017","journal-title":"Journal of Neuroscience"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tins.2022.11.006"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.032410"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroimage.2011.09.015"},{"issue":"5","key":"e_1_2_8_18_1","doi-asserted-by":"crossref","DOI":"10.1115\/1.4063272","article-title":"Constitutive modeling of mouse arteries suggests changes in directional coupling and extracellular matrix remodeling that depend on artery type, age, sex, and elastin amounts","volume":"146","author":"Kailash K. A.","year":"2024","journal-title":"Journal of Biomechanical Engineering"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-510X(98)00288-3"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-023-01148-0"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mrm.1910340303"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00683.2017"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-020-02215-w"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00453.2023"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-8993(01)02786-X"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/30522"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0000000000003760"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2023.1201982"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(98)00080-3"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00592.2023"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.702832"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116224"},{"issue":"6","key":"e_1_2_8_33_1","first-page":"1310","article-title":"N4ITK: Improved N3 bias correction","volume":"29","author":"Tustison N. J.","year":"2010","journal-title":"Institute of Electrical and Electronics Engineers Transactions on Medical Imaging"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-87564-6"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.285338"},{"issue":"3","key":"e_1_2_8_36_1","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1113\/EP087453","article-title":"Cerebral and skeletal muscle feed artery vasoconstrictor responses in a mouse model with greater large elastic artery stiffness","volume":"104","author":"Walker A. E.","year":"2019","journal-title":"Experimental Physiology"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2015.10.012"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP093234","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP093234","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP093234","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T09:10:11Z","timestamp":1769505011000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP093234"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,27]]},"references-count":36,"alternative-id":["10.1113\/EP093234"],"URL":"https:\/\/doi.org\/10.1113\/ep093234","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,27]]},"assertion":[{"value":"2025-08-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"EP093234"}}