{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:56:28Z","timestamp":1790877388607,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201021\u20100164"],"award-info":[{"award-number":["APVV\u201021\u20100164"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to assess the relationship between long\u2010term endurance running training and daily variation in testosterone, which is known to diminish during ageing, in a comparative, cross\u2010sectional study. We recruited 45 male participants into four groups [young athletes (YA), young sedentary (YS), master athletes (MA) and elderly sedentary (ES)] and performed anthropometry, strength and ergometry tests. Morning (AM) and afternoon (PM) venous blood samples were collected to evaluate testosterone daily variation, sex hormone binding globulin (SHBG), cortisol and estradiol, and we calculated free testosterone, bioavailable testosterone and the free androgen index. Two\u2010way and three\u2010way ANOVAs followed by Bonferroni's multiple comparisons tests were performed. The free testosterone and free androgen index showed a significant PM decrease in the MA group (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.0009 and 0.0021, respectively), comparable to YA and YS; however, in the ES group there was no difference between AM and PM values (both\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &gt;\u00a00.9999). SHBG levels were significantly increased by both the age (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.0001) and activity (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.0016) factors, leading to a significant decrease in free testosterone, bioavailable testosterone and free androgen index. In the peak power output parameters of ergometry, expressed in both absolute and relative values, MA achieved values comparable to YS, significantly higher than ES (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00.0020 and\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.0001, respectively) and lower than YA (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.0001). In conclusion, long\u2010term endurance running training in elderly men might be associated with the presence of daily variation in free testosterone and higher performance levels in comparison to sedentary counterparts, but it might contribute to age\u2010related changes in SHBG and related testosterone fraction levels.\n                  <\/jats:p>","DOI":"10.1113\/ep093482","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:21:23Z","timestamp":1789467683000},"page":"4620-4635","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Long\u2010term endurance running training and testosterone daily variation in ageing men; a comparative cross\u2010sectional study"],"prefix":"10.1113","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-6882-9305","authenticated-orcid":false,"given":"Barbora","family":"Bartko","sequence":"first","affiliation":[{"name":"Department of Biological and Medical Sciences Faculty of Physical Education and Sport Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2963-7924","authenticated-orcid":false,"given":"\u013dudmila","family":"Oresk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biological and Medical Sciences Faculty of Physical Education and Sport Comenius University in Bratislava  Bratislava Slovakia"},{"name":"Department of Health Technologies Faculty of Pharmacy Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7712-7358","authenticated-orcid":false,"given":"Gabriel","family":"Buzg\u00f3","sequence":"additional","affiliation":[{"name":"Department of Biological and Medical Sciences Faculty of Physical Education and Sport Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1171-1583","authenticated-orcid":false,"given":"Genc","family":"Berisha","sequence":"additional","affiliation":[{"name":"Department of Biological and Medical Sciences Faculty of Physical Education and Sport Comenius University in Bratislava  Bratislava Slovakia"},{"name":"Department of Physical Education Faculty of Natural Sciences Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-9488-4594","authenticated-orcid":false,"given":"Valent\u00edna","family":"Zeman\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Ethology Faculty of Natural Sciences Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9186-7341","authenticated-orcid":false,"given":"Monika","family":"Okuliarov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Ethology Faculty of Natural Sciences Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2712-6805","authenticated-orcid":false,"given":"Michal","family":"Zeman","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology and Ethology Faculty of Natural Sciences Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2324-154X","authenticated-orcid":false,"given":"Milan","family":"Sedliak","sequence":"additional","affiliation":[{"name":"Department of Biological and Medical Sciences Faculty of Physical Education and Sport Comenius University in Bratislava  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1249\/mss.0b013e31814fb402"},{"key":"e_1_2_10_3_1","volume-title":"ACSM's Guidelines for Exercise Testing and Prescription","author":"American College of Sports Medicine","year":"2025"},{"issue":"4","key":"e_1_2_10_4_1","first-page":"373","article-title":"World Medical Association declaration of Helsinki. 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