{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:57:48Z","timestamp":1790877468131,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministerstvo Zdravotnictv\u00ed Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["#NU21\u201001\u201000469"],"award-info":[{"award-number":["#NU21\u201001\u201000469"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Improving adipose tissue (AT) metabolism through exercise might increase production of lipokines from the fatty acid esters of hydroxy fatty acids (FAHFA) family. Although exercise training can increase insulin sensitivity and levels of specific FAHFA in AT and the circulation in humans, the effects of acute exercise on FAHFA levels remain unclear. Previously untrained lean, obese and aged male C57BL\/6J mice were subjected to acute treadmill exercise. AT lipolysis, metabolomic profile and FAHFA levels were analysed at baseline and 0 and 3\u00a0h postexercise. Baseline AT levels of FAHFA, particularly those containing mono\u2010 and polyunsaturated fatty acids, were lower in obese and aged mice compared with lean mice. AT\u2010specific expression of genes related to\n                    <jats:italic>de novo<\/jats:italic>\n                    lipogenesis and lipolysis was reduced, particularly in obese mice, whereas expression of FAHFA hydrolysis\u2010related genes was higher in aged mice. In lean mice, acute exercise increased plasma non\u2010esterified fatty acids and their release from AT explants, which was suppressed in obese and aged counterparts. Only in lean animals, acute exercise led to a time\u2010dependent increase in total FAHFA levels in AT, with the most pronounced increase observed in 13\/12\u2010regioisomers from different FAHFA subfamilies. The effect of acute exercise on AT FAHFA levels was stronger than that of fasting alone, revealing palmitoleic acid\u2010containing FAHFA as the most differentiating regioisomers. In contrast, plasma FAHFA levels were minimally affected in all groups. In conclusion, acute exercise increases FAHFA levels in AT but not in plasma, and this effect appears to be negatively affected by obesity and ageing.\n                  <\/jats:p>","DOI":"10.1113\/ep093561","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T14:40:10Z","timestamp":1784990410000},"page":"4457-4497","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acute exercise\u2010induced lipolysis and increase in levels of fatty acid esters of hydroxy fatty acids in adipose tissue are impaired in obese and aged mice"],"prefix":"10.1113","volume":"111","author":[{"given":"Marko","family":"Mitrovic","sequence":"first","affiliation":[{"name":"Laboratory of Adipose Tissue Biology Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"},{"name":"First Faculty of Medicine Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Horakova","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Riecan","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism of Bioactive Lipids Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Kleinova","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomas","family":"Cajka","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Metabolism Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Rossmeislova","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Center for Research on Nutrition, Metabolism and Diabetes, Third Faculty of Medicine Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Kuda","sequence":"additional","affiliation":[{"name":"Laboratory of Metabolism of Bioactive Lipids Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8534-4002","authenticated-orcid":false,"given":"Martin","family":"Rossmeisl","sequence":"additional","affiliation":[{"name":"Laboratory of Adipose Tissue Biology Institute of Physiology of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0954-6820.1976.tb08218.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.04.038"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlr.2021.100108"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037794"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185993"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2020999118"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2019.158576"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1997.tb02712.x"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.76.6.2253"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2022.12.056"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/87.4.817"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574-022-00641-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.1.4829"},{"issue":"1","key":"e_1_2_10_15_1","first-page":"R46","article-title":"Adipocyte blood flow: Influence of age, anatomic location, and dietary manipulation","volume":"247","author":"Crandall D. 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