{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:25:20Z","timestamp":1786119920034,"version":"build-2736575974"},"reference-count":102,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["405834662"],"award-info":[{"award-number":["405834662"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Eccentric muscle contractions are essential for locomotion and daily activities. Force during these contractions depends on both cross\u2010bridge (XB) and non\u2010cross\u2010bridge (non\u2010XB) elements, including titin, with contributions varying by fibre type and contraction velocity. Despite their importance, mechanisms of force generation during prolonged stretches remain unclear. Chemical XB inhibitors are commonly used to separate XB and non\u2010XB contributions, but their effects have not been systematically compared under identical conditions. We measured eccentric force in single permeabilised fibres from fast\u2010twitch rat extensor digitorum longus (EDL) and slow\u2010twitch soleus (SOL) muscles. Fibres were treated with blebbistatin (Bleb), 2,3\u2010butanedione monoxime (BDM), or\n                    <jats:italic>N<\/jats:italic>\n                    \u2010benzyl\u2010\n                    <jats:italic>p<\/jats:italic>\n                    \u2010toluene sulphonamide (BTS) and stretched from 0.85 to 1.3 times optimal length at three velocities (0.01, 0.1 and 1.0 maximal contraction velocity). XB inhibition produced fibre\u2010type\u2010dependent effects on early\u2010 and late\u2010stretch force (slope\n                    <jats:sub>1<\/jats:sub>\n                    , slope\n                    <jats:sub>2<\/jats:sub>\n                    ) and on end\u2010of\u2010stretch force (\n                    <jats:italic>s<\/jats:italic>\n                    <jats:sub>e<\/jats:sub>\n                    ). Stretch velocity and the type of XB inhibitor influenced\n                    <jats:italic>s<\/jats:italic>\n                    <jats:sub>e<\/jats:sub>\n                    in both SOL and EDL fibres. However, in EDL fibres,\n                    <jats:italic>s<\/jats:italic>\n                    <jats:sub>e<\/jats:sub>\n                    did not differ significantly between XB inhibitors and showed velocity\u2010dependent effects under Bleb and BTS only, but not under BDM. Stretch\u2010induced forces were less reduced than isometric forces, indicating substantial stretch\u2010dependent non\u2010XB contributions. These results show that eccentric force arises from coordinated XB and non\u2010XB mechanisms in a fibre\u2010type\u2010dependent manner and highlight Bleb as an effective tool for probing these contributions.\n                  <\/jats:p>","DOI":"10.1113\/ep093831","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T01:30:53Z","timestamp":1780363853000},"page":"3337-3356","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of chemical myosin II cross\u2010bridge inhibitors on eccentric force production in skinned muscle fibres"],"prefix":"10.1113","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6957-5094","authenticated-orcid":false,"given":"Andr\u00e9","family":"Tomalka","sequence":"first","affiliation":[{"name":"Motion and Exercise Science University of Stuttgart  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven","family":"Weidner","sequence":"additional","affiliation":[{"name":"Motion and Exercise Science University of Stuttgart  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Rode","sequence":"additional","affiliation":[{"name":"Institute of Sport Science, Department of Biomechanics University of Rostock  Rostock Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Siebert","sequence":"additional","affiliation":[{"name":"Motion and Exercise Science University of Stuttgart  Stuttgart Germany"},{"name":"Stuttgart Center for Simulation Science University of Stuttgart  Stuttgart Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1516732113"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1150170"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-201X.1998.tb10694.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-01-00153.1990"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1994.sp020149"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01737994"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-814593-7.00006-2"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.12.185"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)77738-0"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1998.941bj.x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506830102"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-4622(96)02263-6"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1436004"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb734"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00226.2010"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2011.06.006"},{"key":"e_1_2_9_18_1","volume-title":"Statistical power analysis for the behavioral sciences","author":"Cohen J.","year":"1998"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.124370"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2010.02.014"},{"key":"e_1_2_9_21_1","first-page":"191","article-title":"Cross\u2010bridge properties in single intact frog fibers studied by fast stretches","volume":"682","author":"Colombini B.","year":"2010","journal-title":"International Advances in Experimental Medicine and Biology"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-201x.1998.00329.x"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-025-03088-2"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1978.sp012246"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1977.sp011911"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.86.11.1114"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00865.2018"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017403"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms13281"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1249\/00003677-200604000-00002"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2014.00035"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00538402"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00163a036"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-46957-7"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2413883121"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2209441119"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a122717"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.00536"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2016.00483"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0096-4174(18)30128-8"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/173971a0"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/173973a0"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.2142\/biophysico.15.0_111"},{"key":"e_1_2_9_44_1","volume-title":"Skeletal muscle from molecules to movement: A textbook of muscle physiotherapy for sport, exercise and physiotherapy","author":"Jones J. D.","year":"2004"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2017.191"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP285549"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405319200"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2235652100"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5234.293"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00008.2013"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00049.2010"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-004-6060-7"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.099549"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physiol-021317-121234"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiomech.2023.111553"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvab328"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1990.sp018324"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/245154"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00123483"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.248187"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00073.2010"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1977.232.1.C45"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1996.sp021730"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00290.2014"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1080\/10255842.2010.527837"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2002.027375"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-005-2679-2"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.095448"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP278713"},{"issue":"20","key":"e_1_2_9_70_1","first-page":"3629","article-title":"Titin force is enhanced in actively stretched skeletal muscle","volume":"217","author":"Powers K.","year":"2014","journal-title":"Journal of Experimental Biology"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200509364"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1974.sp010613"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2018.05.037"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.10113"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1002\/mus.21744"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00014.2023"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00377.2004"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1995.269.6.C1532"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2009.03.015"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10974-007-9110-0"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi0483357"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14502"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1994.77.2.493"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00031.2010"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0773.1994.tb01365.x"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1080\/23335432.2019.1597643"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP272983"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi026964f"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0130985"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00122119"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1078\/0065-1281-00660"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1998.275.4.H1260"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtbi.2008.08.009"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-023-02794-z"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2016.2497"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00921"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.644981"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.247377"},{"key":"e_1_2_9_99_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.16.7101"},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.00211"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00735.2021"},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-024-02991-4"},{"key":"e_1_2_9_103_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.2.C437"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP093831","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP093831","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP093831","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T19:27:57Z","timestamp":1786044477000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP093831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,1]]},"references-count":102,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1113\/EP093831"],"URL":"https:\/\/doi.org\/10.1113\/ep093831","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,1]]},"assertion":[{"value":"2026-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}