{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:26:11Z","timestamp":1786119971164,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["310030_215274"],"award-info":[{"award-number":["310030_215274"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    TRPM4 is a calcium\u2010activated, voltage\u2010modulated, non\u2010selective cation channel expressed in various tissues, including the heart. In 2016, we reported on a large French family with progressive heart block type I carrying the variant TRPM4 p.I376T. In the present study, the aim was to investigate the consequence of the channel variant TRPM4 p.I376T in cardiac physiology in a newly generated\n                    <jats:italic>Trpm4<\/jats:italic>\n                    knock\u2010in mouse line. Male and female\n                    <jats:italic>Trpm4<\/jats:italic>\n                    knock\u2010in (\n                    <jats:italic>Trpm4<\/jats:italic>\n                    <jats:sup>I376T\/I376T<\/jats:sup>\n                    ) and wild\u2010type mice of different young ages (12, 18, 24 and 36\u00a0weeks old) were phenotyped using surface ECGs. Western blots were performed to quantify TRPM4 protein surface expression in cardiac tissue. Finally, patch\u2010clamp experiments were conducted to quantify the \u2018TRPM4 current\u2019 from freshly isolated ventricular cardiomyocytes. Assessment of cardiac electrophysiology using surface ECGs indicated no significant differences between the two genotypes at any age. Western blot analyses revealed a significant decrease in the highly glycosylated fraction of the TRPM4 protein in\n                    <jats:italic>Trpm4<\/jats:italic>\n                    <jats:sup>I376T\/I376T<\/jats:sup>\n                    hearts compared with wild\u2010type tissues. However, this alteration did not influence the \u2018TRPM4 current\u2019 when comparing\n                    <jats:italic>Trpm4<\/jats:italic>\n                    <jats:sup>I376T\/I376T<\/jats:sup>\n                    and wild\u2010type cardiomyocytes. These results indicate that the TRPM4 variant, TRPM4 p.I376T, does not alter electrical activity in the murine heart at young ages but decreases the amount of highly glycosylated TRPM4 protein expressed in the heart via an unknown mechanism.\n                  <\/jats:p>","DOI":"10.1113\/ep093873","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:08:41Z","timestamp":1777453721000},"page":"3294-3309","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Glycosylation of the murine cardiac channel TRPM4 is altered by the pathogenic p.I376T variant"],"prefix":"10.1113","volume":"111","author":[{"given":"Sabrina","family":"Guichard","sequence":"first","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"di Lorenzo","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Schneiter","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Essers","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prakash","family":"Arullampalam","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8710-0260","authenticated-orcid":false,"given":"Jean\u2010S\u00e9bastien","family":"Rougier","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0465-5138","authenticated-orcid":false,"given":"Hugues","family":"Abriel","sequence":"additional","affiliation":[{"name":"Institute of Biochemistry and Molecular Medicine University of Bern  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.06.021"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.309202"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.15783"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/nargab\/lqae169"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2016.01.052"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0115256"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1988.sp017242"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.673044"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00004.2010"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-024-01886-1"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-54134-z"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-015-0501-x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI38292"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)00719-5"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054131"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCGENETICS.109.930867"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302835"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI41348"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M305127200"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-003-1221-x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22073401"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13020258"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21599"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2014.00052"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi100115a"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehac354"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.530584"}],"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP093873","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/full-xml\/10.1113\/EP093873","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/EP093873","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T19:28:08Z","timestamp":1786044488000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/EP093873"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,29]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1113\/EP093873"],"URL":"https:\/\/doi.org\/10.1113\/ep093873","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,29]]},"assertion":[{"value":"2026-03-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}