{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:58:02Z","timestamp":1790877482964,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Diabetes mellitus is a major metabolic disorder closely associated with oxidative stress and male reproductive dysfunction. Humanin, a mitochondria\u2010derived peptide, has been reported to exert cytoprotective, anti\u2010apoptotic and antioxidant effects in various disease models; however, its role in diabetes\u2010induced testicular damage remains unclear. This study aimed to investigate the effects of humanin on oxidative stress parameters in seminal vesicle fluid and histopathological alterations in testicular tissue in a streptozotocin (STZ)\u2010induced diabetic mouse model. A total of 40 adult male Balb\/C mice were randomly assigned into four groups: Control, Humanin (HN), STZ, and STZ+HN (\n                    <jats:italic>n<\/jats:italic>\n                    \u00a0=\u00a010 per group). Diabetes was induced via intraperitoneal administration of STZ (150\u00a0mg\/kg). Humanin (4\u00a0mg\/kg) was administered intraperitoneally for 15 days following diabetes induction. Total antioxidant status (TAS), total oxidant status (TOS), and glutathione (GSH) levels in seminal vesicle fluid were measured using ELISA. Testicular tissues were evaluated histopathologically and histomorphometrically. STZ\u2010induced diabetes resulted in a significant decrease in TAS and GSH levels and an increase in TOS levels in seminal vesicle fluid (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.05). Humanin treatment significantly increased TAS and GSH levels while reducing TOS levels compared to the STZ group (\n                    <jats:italic>P\u00a0<\/jats:italic>\n                    &lt;\u00a00.05). Histopathological analysis demonstrated seminiferous tubule degeneration, germ cell loss, interstitial oedema, and vascular congestion in diabetic mice, whereas these alterations and associated histomorphometric abnormalities were significantly ameliorated following humanin treatment. Humanin attenuated diabetes\u2010induced oxidative imbalance and testicular histopathological damage in STZ\u2010induced diabetic mice. These findings suggest that humanin may exert protective effects against diabetes\u2010associated testicular injury. Further studies are required to elucidate the underlying molecular mechanisms and validate these findings in broader experimental settings.\n                  <\/jats:p>","DOI":"10.1113\/ep093912","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T06:42:31Z","timestamp":1783924951000},"page":"4498-4506","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Humanin ameliorates diabetes\u2010induced testicular damage in a streptozotocin\u2010induced mouse model"],"prefix":"10.1113","volume":"111","author":[{"given":"Munevver Gizem","family":"Hekim","sequence":"first","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Firat University  Elazig Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nalan","family":"Kaya Tektemur","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine Firat University  Elazig Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5551-4880","authenticated-orcid":false,"given":"Mete","family":"Ozcan","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Medicine Firat University  Elazig 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