{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:06:09Z","timestamp":1785434769845,"version":"3.56.0"},"reference-count":81,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AG\u2010047245"],"award-info":[{"award-number":["R01 AG\u2010047245"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 AG\u2010058607"],"award-info":[{"award-number":["R01 AG\u2010058607"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Intracellular accumulation of hydrogen ions (H\n                    <jats:sup>+<\/jats:sup>\n                    ) and inorganic phosphate (P\n                    <jats:sub>i<\/jats:sub>\n                    ) has temperature\u2010dependent effects on single\u2010fibre contractile function between 10\u00b0C and 30\u00b0C. In vivo, human skeletal muscle temperatures range between 35\u201039\u00b0C, and although contractile function is highly dependent on temperature, the effects of fatigue\u2010inducing [H\n                    <jats:sup>+<\/jats:sup>\n                    ] and [P\n                    <jats:sub>i<\/jats:sub>\n                    ] on contractile mechanics at 37\u00b0C are unknown. Using sinusoidal analysis, the independent and combined effects of these metabolites on cellular and molecular contractile function were determined at 37\u00b0C in slow\u2010contracting myosin heavy chain (MHC) I and fast\u2010contracting MHC IIA fibres from vastus lateralis muscle of 13 older adults (8 females), in four conditions: maximal calcium activation (\u2018control\u2019; 5\u00a0mM P\n                    <jats:sub>i<\/jats:sub>\n                    , pH 7.0), high P\n                    <jats:sub>i<\/jats:sub>\n                    (30\u00a0mM), low pH (6.2) and fatigue (30\u00a0mM P\n                    <jats:sub>i<\/jats:sub>\n                    and pH 6.2). Specific tension (force\/cross\u2010sectional area, mN\/mm\n                    <jats:sup>2<\/jats:sup>\n                    ) in both fibre types was reduced only in the fatigue condition (20%\u201326%). MHC I fibres had slower cross\u2010bridge kinetics, with fewer or less stiff strongly bound myosin\u2013actin cross\u2010bridges in high P\n                    <jats:sub>i<\/jats:sub>\n                    , low pH and fatigue. The rate of myosin force production was slowest in low pH and fatigue conditions, whereas the myosin detachment rate was most altered by low pH alone. This indicates that during fatigue, high P\n                    <jats:sub>i<\/jats:sub>\n                    moderates the slowing of cross\u2010bridge detachment owing to low pH. In contrast, fatigued MHC IIA fibres had faster cross\u2010bridge kinetics with increased myofilament viscosity. These findings quantify fibre type\u2010specific mechanical and kinetic mechanisms of fatigue in human skeletal muscle at 37\u00b0C, thus advancing our understanding of metabolite\u2010based muscle fatigue in vivo.\n                  <\/jats:p>","DOI":"10.1113\/ep093983","type":"journal-article","created":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T10:03:47Z","timestamp":1781690627000},"page":"3753-3771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phosphate and acidosis cause fibre type\u2010specific changes to cellular and molecular contractile mechanics at 37\u00b0C in skeletal muscle from older adults"],"prefix":"10.1113","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9171-0272","authenticated-orcid":false,"given":"Brent A.","family":"Momb","sequence":"first","affiliation":[{"name":"Department of Kinesiology University of Massachusetts Amherst  Amherst Massachusetts 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