{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:58:06Z","timestamp":1790877486261,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["physoc.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Balance and gaze rely on the rapid and accurate detection and signalling of head movements by vestibular type I and type II hair cells. Signal transduction and transmission involve several types of ion channels, which are acquired progressively during hair cell differentiation and whose identity is known only in part. In the present study, we adopted a multi\u2010omics approach to identify the molecular nature of ion channels expressed by chicken vestibular hair cells between embryonic day 15 and embryonic day 21, the day of hatching, when both type I and II hair cells appear functionally mature. Type I hair cells are characterized by the expression of a low\u2010voltage\u2010activated outward rectifying K\n                    <jats:sup>+<\/jats:sup>\n                    current, termed\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>KL<\/jats:sub>\n                    . In adult mice,\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>KL<\/jats:sub>\n                    is carried by the K\n                    <jats:sub>V<\/jats:sub>\n                    1.8 \u03b1\u2010subunit, although it is still debatable whether the K\n                    <jats:sub>V<\/jats:sub>\n                    7.4 \u03b1\u2010subunit contributes to\n                    <jats:italic>I<\/jats:italic>\n                    <jats:sub>KL<\/jats:sub>\n                    in immature mouse type I hair cells. In this study, no effect of linopirdine, a specific blocker of K\n                    <jats:sub>V<\/jats:sub>\n                    7 channels, was found in either immature or mature type I or II hair cells. Furthermore, whole\u2010organ RT\u2010PCR and transcriptome analysis showed no expression of the K\n                    <jats:sub>V<\/jats:sub>\n                    7.4 \u03b1\u2010subunit transcript, while detecting the transcript for the K\n                    <jats:sub>V<\/jats:sub>\n                    1.8 subunit. Its expression in type I and II hair cells was finally confirmed by patch\u2010sequencing experiments. Further analysis of the transcriptomes of individual hair cells revealed the presence of transcripts for several other \u03b1 and auxiliary subunits of Na\n                    <jats:sup>+<\/jats:sup>\n                    , K\n                    <jats:sup>+<\/jats:sup>\n                    and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    channels, which are, presumably, responsible for the ionic currents recorded in both birds and mammals.\n                  <\/jats:p>","DOI":"10.1113\/ep094078","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T08:24:34Z","timestamp":1789460674000},"page":"4894-4914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010omics analysis of ion channels expressed by chicken embryo vestibular type I and type II hair cells"],"prefix":"10.1113","volume":"111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2024-8594","authenticated-orcid":false,"given":"Giulia","family":"Cheli","sequence":"first","affiliation":[{"name":"Department of Brain and Behavioral Sciences University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Giunta","sequence":"additional","affiliation":[{"name":"Department of Brain and Behavioral Sciences University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Stella","sequence":"additional","affiliation":[{"name":"Department of Brain and Behavioral Sciences University of Pavia  Pavia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Guidi","sequence":"additional","affiliation":[{"name":"Center for Omics Sciences IRCCS San Raffaele Institute  Milan Italy"},{"name":"Vita\u2010Salute San Raffaele University  Milan 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Tavakoli A. Kulasooriya S. Liu H. Tu S. Bloom C. Li Y. Johnson T. D. Zuo J. Tao L. Kachar B. &He D. Z.(2025).The dual molecular identity of vestibular kinocilia: Bridging structural and functional traits of primary and motile cilia.BioRxiv: The Preprint Server for Biology.https:\/\/doi.org\/10.1101\/2025.04.16.647417","DOI":"10.1101\/2025.04.16.647417"},{"key":"e_1_2_11_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00522-4"},{"issue":"5","key":"e_1_2_11_68_1","first-page":"387","article-title":"Characterization of human Kv4.2 mediating a rapidly\u2010inactivating transient voltage\u2010sensitive K+ current","volume":"6","author":"Zhu X. 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