{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:53:52Z","timestamp":1772895232322,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,5,4]],"date-time":"2004-05-04T00:00:00Z","timestamp":1083628800000},"content-version":"vor","delay-in-days":1038,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:p>Effects of prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) on exocytosis of mucin were studied in mucous cells isolated from guinea\u2010pig antrum using video\u2010microscopy. Stimulation with PGE<jats:sub>2<\/jats:sub> elicited a sustained increase in the frequency of exocytotic events in a dose\u2010dependent manner, which was under regulation by both Ca<jats:sup>2+<\/jats:sup> and cAMP. Stimulation with a selective prostanoid EP4 receptor agonist (ONO\u2010AEI\u2010329, 10 \u03bcM), which activates cAMP signals, elicited a sustained increase in the frequency of exocytotic events (30% of that evoked by 1 \u03bcM PGE<jats:sub>2<\/jats:sub>). Stimulation with an EP1 agonist (17\u2010P\u2010T\u2010PGE<jats:sub>2<\/jats:sub>, 1 \u03bcM), which activates Ca<jats:sup>2+<\/jats:sup> signals, increased the frequency of exocytotic events to a lesser extent (5% of that evoked by 1 \u03bcM PGE<jats:sub>2<\/jats:sub>), while addition of an EP1 antagonist (ONO\u20108713, 10 \u03bcM) decreased the frequency of exocytotic events (approximately 40% of that evoked by 1 \u03bcM PGE<jats:sub>2<\/jats:sub>). However, addition of the EP1 agonist potentiated the frequency of exocytotic events evoked by the EP4 agonist or forskolin (which elevates cAMP levels) and increased the sensitivity of the exocytotic events to forskolin. These results suggest that the Ca<jats:sup>2+<\/jats:sup> signal activated via the EP1 receptor potentiates the cAMP\u2010regulated exocytotic events activated via the EP4 receptor during PGE<jats:sub>2<\/jats:sub> stimulation, by increasing the sensitivity of the exocytotic response to cAMP. In conclusion, exocytotic events in PGE<jats:sub>2<\/jats:sub>\u2010stimulated antral mucous cells were regulated by interactions between EP1 and EP4 receptors.<\/jats:p>","DOI":"10.1113\/eph8602160","type":"journal-article","created":{"date-parts":[[2003,2,11]],"date-time":"2003-02-11T16:35:05Z","timestamp":1044981305000},"page":"451-460","source":"Crossref","is-referenced-by-count":28,"title":["EP1 and EP4 Receptors Mediate Exocytosis Evoked by Prostaglandin E<sub>2<\/sub> in Guinea\u2010Pig Antral Mucous Cells"],"prefix":"10.1113","volume":"86","author":[{"given":"Atsuko","family":"Ohnishi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikao","family":"Shimamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken\u2010ichi","family":"Katsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sigenori","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yusuke","family":"Imai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Nakahari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,4]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Feph8602160","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Feph8602160","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/eph8602160","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:22:26Z","timestamp":1700418146000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/eph8602160"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,7]]}},"alternative-id":["10.1113\/eph8602160"],"URL":"https:\/\/doi.org\/10.1113\/eph8602160","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7]]}}}