{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T00:04:42Z","timestamp":1772064282448,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,5,4]],"date-time":"2004-05-04T00:00:00Z","timestamp":1083628800000},"content-version":"vor","delay-in-days":1038,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2001,7]]},"abstract":"<jats:p>In vitro evidence suggests that the \u2018recycling\u2019 of K<jats:sup>+<\/jats:sup> ions through luminal K<jats:sup>+<\/jats:sup> channels in the thick ascending limb of the loop of Henle (TALH) is essential for the normal operation of the luminal Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u20102Cl<jats:sup>\u2212<\/jats:sup> co\u2010transporter. In the present study these channels were investigated in vivo by perfusing superficial loops of Henle in anaesthetised rats with and without the K<jats:sup>+<\/jats:sup> channel blocker barium. Using a standard perfusate, intraluminal barium (5 mmol l<jats:sup>\u22121<\/jats:sup>) reduced sodium reabsorption (J<jats:sub>Na<\/jats:sub>) from 1887 \u00b1 50 to 1319 \u00b1 53 pmol min<jats:sup>\u22121<\/jats:sup> (P &lt; 0.001). When the experiment was repeated using a low\u2010Na<jats:sup>+<\/jats:sup> perfusate, designed to inhibit reabsorption in the pars recta (the initial segment of the loop of Henle), a similar reduction in J<jats:sub>Na<\/jats:sub> was observed (from 698 \u00b1 47 to 149 \u00b1 23 pmol min<jats:sup>\u22121<\/jats:sup>, P &lt; 0.001), strongly suggesting that the effect of barium is localised to the TALH. The magnitude of the reduction in J<jats:sub>Na<\/jats:sub> during blockade of K<jats:sup>+<\/jats:sup> channels confirms the importance of K<jats:sup>+<\/jats:sup> recycling in facilitating Na<jats:sup>+<\/jats:sup> reabsorption in the TALH in vivo. However, the reduction in J<jats:sub>Na<\/jats:sub> was not associated with a fall in the K<jats:sup>+<\/jats:sup> concentration of the fluid collected at the early distal tubule. When bumetanide, an inhibitor of the Na<jats:sup>+<\/jats:sup>\u2010K<jats:sup>+<\/jats:sup>\u20102Cl<jats:sup>\u2212<\/jats:sup> co\u2010transporter, was included in the low\u2010Na<jats:sup>+<\/jats:sup> perfusate, net K<jats:sup>+<\/jats:sup> secretion was observed. Addition of barium to this perfusate reduced, but did not abolish, the secretion, suggesting that bumetanide\u2010induced K<jats:sup>+<\/jats:sup> secretion results partly from paracellular transport.<\/jats:p>","DOI":"10.1113\/eph8602210","type":"journal-article","created":{"date-parts":[[2003,2,11]],"date-time":"2003-02-11T16:35:05Z","timestamp":1044981305000},"page":"469-474","source":"Crossref","is-referenced-by-count":16,"title":["Effects of the Potassium Channel Blocker Barium on Sodium and Potassium Transport in the Rat Loop of Henle <i>In Vivo<\/i>"],"prefix":"10.1113","volume":"86","author":[{"given":"S. J.","family":"Walter","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. G.","family":"Shirley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. J.","family":"Folkerd","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. J.","family":"Unwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,4]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Feph8602210","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Feph8602210","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/eph8602210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:22:24Z","timestamp":1700418144000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/eph8602210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,7]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2001,7]]}},"alternative-id":["10.1113\/eph8602210"],"URL":"https:\/\/doi.org\/10.1113\/eph8602210","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,7]]}}}