{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:07:32Z","timestamp":1767650852033},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,5,4]],"date-time":"2004-05-04T00:00:00Z","timestamp":1083628800000},"content-version":"vor","delay-in-days":369,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:p>Maintaining the extracellular K<jats:sup>+<\/jats:sup> concentration ([K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub>) between 15 and 60 mM induced oscillations in the intracellular Ca<jats:sup>2+<\/jats:sup> concentration ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) in rat submandibular acinar cells during stimulation with acetylcholine (ACh, 1 \u03bcM). These [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations were also induced by 1 \u03bcM thapsigargin and were inhibited by 50 \u03bcM La<jats:sup>3+<\/jats:sup>, 1 \u03bcM Gd<jats:sup>3+<\/jats:sup>, or the removal of extracellular Ca<jats:sup>2+<\/jats:sup>, indicating that the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations were generated by store\u2010operated Ca<jats:sup>2+<\/jats:sup> entry (SOC). The frequency of the ACh\u2010evoked [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations increased from 0.8 to 2.3 mHz as [K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub> was increased from 15 to 50 mM. TEA (an inhibitor of K<jats:sup>+<\/jats:sup> channels) also induced [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations at [K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub> of 4.5 or 7.5 mM in ACh\u2010stimulated cells. These data suggest that depolarization causes [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> to oscillate in ACh\u2010stimulated submandibular acinar cells. Pertussis toxin (PTX, an inhibitor of G proteins) caused [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> to be sustained at a high level in ACh\u2010stimulated cells at 25 mM or 60 mM [K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub>. This suggests that the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations are generated by a periodic inactivation of the SOC channels via PTX\u2010sensitive G proteins, which are stimulated by depolarization. Moreover, in the presence of DBcAMP or forskolin which accumulated cAMP the frequency of the [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillations remained constant (approximately 1.2 mHz) when [K<jats:sup>+<\/jats:sup>]<jats:sub>o<\/jats:sub> was maintained in the range 25\u201060 mM. Based on these observations in ACh\u2010stimulated submandibular acinar cells, we conclude that depolarization stimulates the PTX\u2010sensitive G proteins, which inactivate the SOC channels periodically ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> oscillation), while hyperpolarization or PTX inhibits the G proteins, maintaining the activation of the SOC channels. Accumulation of cAMP is likely to modulate the PTX\u2010sensitive G proteins.<\/jats:p>","DOI":"10.1113\/eph8802566","type":"journal-article","created":{"date-parts":[[2003,7,16]],"date-time":"2003-07-16T10:11:12Z","timestamp":1058350272000},"page":"369-379","source":"Crossref","is-referenced-by-count":14,"title":["[Ca<sup>2+<\/sup>]<sub>i<\/sub> Oscillations Induced by High [K<sup>+<\/sup>]<sub>o<\/sub> in Acetylcholine\u2010Stimulated Rat Submandibular Acinar Cells: Regulation by Depolarization, cAMP and Pertussis Toxin"],"prefix":"10.1113","volume":"88","author":[{"given":"Hideyo","family":"Yoshida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshinori","family":"Marunaka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Nakahari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,4]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Feph8802566","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Feph8802566","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/eph8802566","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T19:10:29Z","timestamp":1700248229000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/eph8802566"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2003,5]]}},"alternative-id":["10.1113\/eph8802566"],"URL":"https:\/\/doi.org\/10.1113\/eph8802566","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5]]}}}