{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T04:36:41Z","timestamp":1776400601350,"version":"3.51.2"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1986,4,7]],"date-time":"1986-04-07T00:00:00Z","timestamp":513216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Physiol"],"published-print":{"date-parts":[[1986,4,7]]},"abstract":"<jats:p>A technique is described, involving tissue dissociation and micro\u2010dissection, for the isolation of interlobular ducts from the pancreas of copper\u2010deficient rats. The average length and outside diameter of the isolated ducts were 589\u00b70\u00b118\u00b76 and 78\u00b71\u00b11\u00b76 \u00b5m (mean \u00b1 S.E.M., <jats:italic>n<\/jats:italic> = 425) respectively. Between twenty and fifty ducts could be obtained from each pancreas. Frequently, the smaller intralobular ducts, which had outside diameters of between 15 and 25 \u00b5m, were observed as branches of the interlobular ducts. Light and electron microscopy showed that the isolated ducts were structurally intact, and that the epithelial cells possessed all the typical ultrastructural features of duct cells within the gland of copper\u2010replete rats. The isolated ducts consumed oxygen at a rate of 2\u00b727\u00b10\u00b755 ml O<jats:sub>2<\/jats:sub>\/min.100 g wet weight duct epithelium (<jats:italic>n<\/jats:italic> = 6). The concentrations of ATP, ADP and AMP in the ducts were 3\u00b778\u00b10\u00b781, 0\u00b768\u00b10\u00b719 and 0\u00b741\u00b10\u00b713 mmol\/l duct epithelium (<jats:italic>n<\/jats:italic> = 8) respectively. These data give values for ATP:ADP and ATP:AMP ratios of 5\u00b76:1 and 9\u00b72:1 respectively, and an energy charge of 0\u00b785\u00b10\u00b701 (<jats:italic>n<\/jats:italic> = 8) suggesting that the epithelial cells are healthy and in a stable metabolic state. In the presence of the phosphodiesterase inhibitor 3\u2010isobutyl\u20101\u2010methylxanthine (0\u00b767 mM), the basal concentration of cyclic AMP in the isolated ducts was 17\u00b74\u00b10\u00b77 \u00b5mol\/1 duct epithelium (<jats:italic>n<\/jats:italic> = 3). Secretin (0\u00b71 nM\u20101 \u00b5M) caused a dose\u2010related increase in cyclic AMP content up to a maximum of 376\u00b70\u00b185\u00b73 \u00b5mol\/l duct epithelium (<jats:italic>n<\/jats:italic> = 4). This indicates that the epithelial cells possess secretin receptors, and that these receptors can be functionally linked to adenylate cyclase.<\/jats:p>","DOI":"10.1113\/expphysiol.1986.sp002982","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T16:16:37Z","timestamp":1418746597000},"page":"249-265","source":"Crossref","is-referenced-by-count":33,"title":["ISOLATION OF DUCTS FROM THE PANCREAS OF COPPER\u2010DEFICIENT RATS"],"prefix":"10.1113","volume":"71","author":[{"given":"S.","family":"Arkle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"Cullen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. E.","family":"Argent","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1986,4,7]]},"container-title":["Quarterly Journal of Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1986.sp002982","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1986.sp002982","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T18:57:21Z","timestamp":1697914641000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1986.sp002982"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,4,7]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1986,4,7]]}},"alternative-id":["10.1113\/expphysiol.1986.sp002982"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1986.sp002982","archive":["Portico"],"relation":{},"ISSN":["0144-8757"],"issn-type":[{"value":"0144-8757","type":"print"}],"subject":[],"published":{"date-parts":[[1986,4,7]]}}}