{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T09:12:02Z","timestamp":1697965922698},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[1988,11,12]],"date-time":"1988-11-12T00:00:00Z","timestamp":595296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Exp Physiol"],"published-print":{"date-parts":[[1988,11,12]]},"abstract":"<jats:p>The effects of the anion transport inhibitor DIDS (4,4'\u2010diisothiocyano\u2010stilbene\u20102,2'\u2010 disulphonate) on fluid reabsorption by the rat proximal convoluted tubule were investigated by the stationary microperfusion (split\u2010drop) method. Addition of 10<jats:sup>-5<\/jats:sup> mol l<jats:sup>\u22121<\/jats:sup> DIDS to luminal and capillary perfusates in the presence of peritubular bicarbonate (25 mmol l<jats:sup>\u22121<\/jats:sup>) increased the reabsorptive half\u2010time (<jats:italic>t<\/jats:italic><jats:sub>\u00bd<\/jats:sub>) from 35\u00b71 \u00b1 4\u00b75 to 57\u00b70 \u00b1 6\u00b72 s, corresponding to a decrease in fluid reabsorption (<jats:italic>J<\/jats:italic><jats:sub>v<\/jats:sub>) from 1\u00b733 \u00b1 0\u00b706 to 0\u00b789 \u00b1 0\u00b709 nl min<jats:sup>\u22121<\/jats:sup> mm<jats:sup>\u22121<\/jats:sup>. Omission of bicarbonate <jats:italic>per se<\/jats:italic> from the peritubular capillaries reduced <jats:italic>J<\/jats:italic><jats:sub>v<\/jats:sub> to 0\u00b741 \u00b1 0\u00b703 nl min<jats:sup>\u22121<\/jats:sup> mm<jats:sup>\u22121<\/jats:sup> and this reabsorptive flux was completely inhibited by the addition of 2 mmol l<jats:sup>\u22121<\/jats:sup> NaCN to both the luminal and peritubular perfusates. In the absence of bicarbonate, the addition of DIDS at either 10<jats:sup>\u22124<\/jats:sup> or 10<jats:sup>\u22125<\/jats:sup> mol l<jats:sup>\u22121<\/jats:sup> to both lumen and capillary perfusates had no significant effect on either <jats:italic>t<\/jats:italic><jats:sub>\u00bd<\/jats:sub> or <jats:italic>J<\/jats:italic><jats:sub>v<\/jats:sub>. These results show that the inhibitory action of DIDS on fluid reabsorption is dependent on the presence of bicarbonate. However, the proximal tubule also reabsorbs water in the absence of bicarbonate by a mechanism that is dependent on cellular metabolism, but is insensitive to DIDS at the concentrations used in this study.<\/jats:p>","DOI":"10.1113\/expphysiol.1988.sp003229","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T15:54:26Z","timestamp":1418745266000},"page":"951-958","source":"Crossref","is-referenced-by-count":1,"title":["THE EFFECT OF DIDS ON FLUID REABSORPTION FROM THE PROXIMAL CONVOLUTED TUBULE OF THE RAT: DEPENDENCE ON THE PRESENCE OF BICARBONATE"],"prefix":"10.1113","volume":"73","author":[{"given":"S. J.","family":"White","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. L.","family":"Greenwood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Green","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1988,11,12]]},"container-title":["Quarterly Journal of Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1988.sp003229","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1988.sp003229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T04:57:31Z","timestamp":1697864251000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1988.sp003229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1988,11,12]]}},"alternative-id":["10.1113\/expphysiol.1988.sp003229"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1988.sp003229","archive":["Portico"],"relation":{},"ISSN":["0144-8757"],"issn-type":[{"value":"0144-8757","type":"print"}],"subject":[],"published":{"date-parts":[[1988,11,12]]}}}