{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T23:15:07Z","timestamp":1722035707665},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1990,3,1]],"date-time":"1990-03-01T00:00:00Z","timestamp":636249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Physiology"],"published-print":{"date-parts":[[1990,3]]},"abstract":"<jats:p>The effects of a synthetic and an endogenous steroid were studied on the GABAA receptors of isolated mouse spinal neurones, maintained in culture. Low doses of alphaxalone reversibly increased GABA\u2010evoked whole\u2010cell currents. Alphaxalone at higher doses (10\u201050 microM), when pressure ejected onto spinal neurones, also directly evoked a membrane chloride current. Such currents were reversibly suppressed by bicuculline (a GABAA antagonist) and enhanced by phenobarbitone. 5 beta\u2010Pregnan\u20103 alpha\u2010ol\u201020\u2010one, a progesterone metabolite, dose\u2010dependently potentiated the amplitude of GABA\u2010evoked whole\u2010cell currents. The mechanism of potentiation was examined at the single\u2010channel level using outside\u2010out patches from spinal neurones. The main action of the steroid on the GABAA receptor appears to be similar to that found for barbiturates, in that they prolonged GABA\u2010activated bursts of channel openings. Bemegride had an antagonistic action on the GABAA receptor, suppressing both GABA\u2010 and pentobarbitone\u2010evoked whole\u2010cell currents to similar extents.<\/jats:p>","DOI":"10.1113\/expphysiol.1990.sp003394","type":"journal-article","created":{"date-parts":[[2014,12,16]],"date-time":"2014-12-16T14:45:58Z","timestamp":1418741158000},"page":"199-209","source":"Crossref","is-referenced-by-count":18,"title":["Actions of steroids and bemegride on the GABAA receptor of mouse spinal neurones in culture"],"prefix":"10.1113","volume":"75","author":[{"given":"DK","family":"Mistry","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"GA","family":"Cottrell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,3]]},"container-title":["Experimental Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fexpphysiol.1990.sp003394","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/expphysiol.1990.sp003394","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T10:24:37Z","timestamp":1697970277000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/expphysiol.1990.sp003394"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,3]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,3]]}},"alternative-id":["10.1113\/expphysiol.1990.sp003394"],"URL":"https:\/\/doi.org\/10.1113\/expphysiol.1990.sp003394","archive":["Portico"],"relation":{},"ISSN":["0958-0670","1469-445X"],"issn-type":[{"value":"0958-0670","type":"print"},{"value":"1469-445X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,3]]}}}